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by Jmitra at 10-29-2021, 08:50 PM
<p>How reliable is the TRI-DOT HIV test? Is there a case where a person is infected but the TRI-DOT showed a negative result? Recently I have gotten to know through my diagnostic lab colleague about this. he is currently using&nbsp;<a href="https://jmitra.co.in/product-details/hiv-rapid-test-kit-tri-dot/">HIV Test Kit</a>&nbsp;provided by a company J Mitra. According to him, the results are good &amp; accurate. just want to suggest.</p>
by jumotakudzwa@gmail.com at 09-24-2021, 08:55 PM
<p>May someone please help me with this:</p><p>
<!--StartFragment-->You observed that the CRISPR knock-out of protein X either promotes or
represses RNA polymerase II transcription, depending on the gene. Which
experiments would you carry out to reveal the mechanism for this
differential activity?<!--EndFragment-->&nbsp;</p><p><br></p><p>Thank you<br></p>
by jumotakudzwa@gmail.com at 09-24-2021, 08:52 PM
<!--StartFragment-->You observed that the CRISPR knock-out of protein X either promotes or
represses RNA polymerase II transcription, depending on the gene. Which
experiments would you carry out to reveal the mechanism for this
differential activity?<!--EndFragment-->

<p><br></p>
by Cayenne at 08-03-2021, 07:12 PM
What problems did you face when you tried to commercialize your scientific discoveries or inventions from the laboratory?
by Drig at 06-30-2021, 07:30 AM
Hello!

I'm working on a personal project to convert a Guanine into an Adenine in this specific albinism causing allele: https://www.ncbi.nlm.nih.gov/gene?Db=gen...;Term=4948

The mutation is at 2324 in the code.
My plan is to use a double nickase dCas9(D10A) to bring the AncBE4max to that site and convert the Guanine into Adenine while in-vivo.

I took inspiration from this article in particular: https://www.nature.com/articles/jhg2009130?proof=t

And this one: https://www.mdpi.com/2073-4409/9/7/1690/htm

As well as this article: https://www.nature.com/articles/s41467-019-11514-0

I haven't yet built a plasmid from scratch&nbsp;and am trying to use GenSmart Design to do so, but am not yet able to figure out how to delete things from the prebuilt plasmid sections. I am hoping to get some aide into what programs I should be using to build my plasmid as well as any know-how into how this works and if it can work.

Using the sgrna design tools online with the basic PAM of NGG, I haven't been able to find sites close enough to the edit zone to seem viable. I am trying to keep it below a span of 40 nucleotides, but I also don't have a good idea of what I'm doing. Any help with explaining how I should be going about this would be wonderful.

Due to certain personal circumstances, I am unable to continue my education at university and am trying to find other ways to continue my education into this field. Any tips for that would also be greatly appreciated.

Godspeed,

- Drig
by jstarr001 at 06-25-2021, 12:53 AM
<p>The title says it all.&nbsp; I need to find a lab already setup to do routine ELISA assays for Akt phosphorylation in PBMCs.&nbsp; Something along the lines of the ThermoFisher Akt(pS473) assay kits or equivalent.&nbsp; All the labs I have canvassed are unfamiliar and require&nbsp;method transfer and validation which turns out to be&nbsp;triple the cost of running the assays themselves.&nbsp; This would be non-GLP, could be academic or commercial lab and would initially be about 300 samples in duplicate.</p><p><br></p><p>Thanks in advance</p><p><br></p><p>John&nbsp;</p><p><br></p>
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by taniamahmood38@yahoo.com at 05-29-2021, 06:07 AM
Hi, anyone please make me clear if it is possible to utilize the magnetic bead-based for extracting DNA from bird feathers? If any kind of literature or protocol is available on it then kindly share the link also.



Thanks in advance.
by franklinpulikkottil at 05-28-2021, 07:01 AM
<!--StartFragment--><p style="text-align: justify; margin: 0px; padding: 0px; font-family: Tahoma, Verdana, Arial, sans-serif; font-size: 14px; color: rgb(51, 51, 51); font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;"><span style="color: rgb(26, 26, 27); font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">I am looking to build a research community focused&nbsp;on&nbsp;the field of biomedical research that works actively for a social cause in India. Our idea is to form a community that dedicates its effort in carrying out research focusing on providing effective solutions for key problems in biotechnology/biomedical research, (currently our focus is on building up a research community for fighting emerging diseases and pand<span id="sceditor-start-marker" class="sceditor-selection sceditor-ignore" style="display: none; line-height: 0;"> </span><span id="sceditor-end-marker" class="sceditor-selection sceditor-ignore" style="display: none; line-height: 0;"> </span>emics) which very few people actually work on with pure motivation in creating a translatable solution to a problem, rather than in generating publication and maintaining profile ( I know this is an integral part of research career no offense, but motivation priority matters here a lot). As the community grows&nbsp;we have to plan and work&nbsp;on how to work together, channelize funding, and set up a registered organization.&nbsp;Anybody interested/motivated to be a part of the community for this cause are welcome to join and let the community grow! Any ideas/suggestions are highly appreciated.</span></p><p style="margin: 0px; padding: 0px; font-family: Tahoma, Verdana, Arial, sans-serif; font-size: 14px; color: rgb(51, 51, 51); font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;"><span style="color: rgb(26, 26, 27); font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">ThanksSmile</span><br></p><p style="margin: 0px; padding: 0px; font-family: Tahoma, Verdana, Arial, sans-serif; font-size: 14px; color: rgb(51, 51, 51); font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;"><span style="color: rgb(26, 26, 27); font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">Franklin&nbsp;</span></p><p style="margin: 0px; padding: 0px; font-family: Tahoma, Verdana, Arial, sans-serif; font-size: 14px; color: rgb(51, 51, 51); font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;"><span style="color: rgb(26, 26, 27); font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;">email:franklinpulikkottil95@gmail.com (feel free to contact here)</span></p><div style="min-height: 1.25em; color: rgb(51, 51, 51); font-family: Tahoma, Verdana, Arial, sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;"><p style="margin: 0px; padding: 0px; font-family: Tahoma, Verdana, Arial, sans-serif; font-size: 14px; color: rgb(51, 51, 51);"><span style="color: rgb(26, 26, 27); font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial; float: none; display: inline !important;"></span>&nbsp;</p></div><!--EndFragment-->

<p class="sceditor-nlf"><br></p>
by apex128 at 05-24-2021, 01:56 PM
Hello,

I want to learn how to genetically modify an insect so that it may only produce sterile offspring. How would I do this? Is this possible to do at home without a lab?

Thanks
by buildloop at 05-08-2021, 02:43 PM
<p><meta charset="UTF-8"></p><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none; margin: 0px; padding: 0px 0px 0.25em; border: 0px; font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-stretch: inherit; line-height: inherit; vertical-align: baseline; caret-color: rgb(26, 26, 27); color: rgb(26, 26, 27);">Hi All,</p><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none; margin: 0px; padding: 0.8em 0px 0.25em; border: 0px; font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-stretch: inherit; line-height: inherit; vertical-align: baseline; caret-color: rgb(26, 26, 27); color: rgb(26, 26, 27);">I'm the developer of the "Drug Bible" and other apps such as Smart Pill ID ( an AI powered pill identifier).</p><p class="" style=""><br><br></p><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none; margin: 0px; padding: 0.8em 0px 0.25em; border: 0px; font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-stretch: inherit; line-height: inherit; vertical-align: baseline; caret-color: rgb(26, 26, 27); color: rgb(26, 26, 27);">Available on the<span class="Apple-converted-space">&nbsp;</span><a href="https://apps.apple.com/us/app/drug-bible-prescription-guide/id1564679108" class="_3t5uN8xUmg0TOwRCOGQEcU" rel="noopener nofollow ugc" target="_blank" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline; color: var(--newCommunityTheme-linkText); text-decoration: underline;">App Store</a><span class="Apple-converted-space">&nbsp;</span>and<span class="Apple-converted-space">&nbsp;</span><a href="https://play.google.com/store/apps/details?id=com.buildloop.drugbible" class="_3t5uN8xUmg0TOwRCOGQEcU" rel="noopener nofollow ugc" target="_blank" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline; color: var(--newCommunityTheme-linkText); text-decoration: underline;">Play Store</a>.</p><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none; margin: 0px; padding: 0.8em 0px 0.25em; border: 0px; font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-stretch: inherit; line-height: inherit; vertical-align: baseline; caret-color: rgb(26, 26, 27); color: rgb(26, 26, 27);">I worked with users in the FDA, NIH, and other health organizations to complete the ultimate drug guide. This handy tool has all the information you will ever need as a consumer, prescriber, or researcher. The Drug Bible has information on over 100,000 prescription and over the counter drugs, both brand name and generics.</p><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none; margin: 0px; padding: 0.8em 0px 0.25em; border: 0px; font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-stretch: inherit; line-height: inherit; vertical-align: baseline; caret-color: rgb(26, 26, 27); color: rgb(26, 26, 27);">Quickly search for drugs or by application to view drug images, labels, documents (research, reviews, letters, and others), as well as clinical and consumer information. Consumer information includes general information, side effects, news, and drug prices. Clinical information includes the following information:</p><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none; margin: 0px; padding: 0.8em 0px 0.25em; border: 0px; font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-stretch: inherit; line-height: inherit; vertical-align: baseline; caret-color: rgb(26, 26, 27); color: rgb(26, 26, 27);"><br></p><ul class="_33MEMislY0GAlB78wL1_CR" style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none; margin: 4px 0px 4px 8px; padding: 0px; border: 0px; font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-stretch: inherit; line-height: inherit; vertical-align: baseline; list-style: disc outside; caret-color: rgb(26, 26, 27); color: rgb(26, 26, 27);"><li class="_3gqTEjt4x9UIIpWiro7YXz" style="margin: 0.4em 1em; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;"><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;">Adverse Reactions</p></li><li class="_3gqTEjt4x9UIIpWiro7YXz" style="margin: 0.4em 1em; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;"><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;">Clinical Pharmacology</p></li><li class="_3gqTEjt4x9UIIpWiro7YXz" style="margin: 0.4em 1em; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;"><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;">Contraindications</p></li><li class="_3gqTEjt4x9UIIpWiro7YXz" style="margin: 0.4em 1em; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;"><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;">Description</p></li><li class="_3gqTEjt4x9UIIpWiro7YXz" style="margin: 0.4em 1em; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;"><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;">Dosage and Administration</p></li><li class="_3gqTEjt4x9UIIpWiro7YXz" style="margin: 0.4em 1em; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;"><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;">Drug Interactions</p></li><li class="_3gqTEjt4x9UIIpWiro7YXz" style="margin: 0.4em 1em; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;"><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;">How Supplied</p></li><li class="_3gqTEjt4x9UIIpWiro7YXz" style="margin: 0.4em 1em; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;"><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;">Indications and Usage</p></li><li class="_3gqTEjt4x9UIIpWiro7YXz" style="margin: 0.4em 1em; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;"><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;">Mechanism of Action</p></li><li class="_3gqTEjt4x9UIIpWiro7YXz" style="margin: 0.4em 1em; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;"><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;">Overdosage</p></li><li class="_3gqTEjt4x9UIIpWiro7YXz" style="margin: 0.4em 1em; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;"><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;">Pediatric Use</p></li><li class="_3gqTEjt4x9UIIpWiro7YXz" style="margin: 0.4em 1em; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;"><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="margin: 0px; padding: 0px; border: 0px; font-family: inherit; font-size: inherit; font-style: inherit; font-variant-caps: inherit; font-weight: inherit; font-stretch: inherit; line-height: inherit; vertical-align: baseline;">Pregnancy</p></li></ul><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none; margin: 0px; padding: 0.8em 0px 0.25em; border: 0px; font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-stretch: inherit; line-height: inherit; vertical-align: baseline; caret-color: rgb(26, 26, 27); color: rgb(26, 26, 27);">Once a medical application has been chosen, you may filter the drugs by Brand Name, Generic Name, Substance Name, Manufacturer Name, Chemical Structure Class, Established Pharmacologic Class, Pharmacological Class, and Mechanism of Action. Drug information is updated monthly.</p><p class="_1qeIAgB0cPwnLhDF9XSiJM" style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none; margin: 0px; padding: 0.8em 0px 0px; border: 0px; font-family: &quot;Noto Sans&quot;, Arial, sans-serif; font-size: 14px; font-stretch: inherit; line-height: inherit; vertical-align: baseline; caret-color: rgb(26, 26, 27); color: rgb(26, 26, 27);">Any and all feedback is much appreciated!</p><br><p></p>
by jheny46 at 05-07-2021, 11:42 AM
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<!--StartFragment--><table><tbody><tr><td><br> &nbsp;&nbsp;&nbsp;&nbsp;Jinan Precision CNC Machinery Co.,Ltd is a professional manufacturer of laser machine and CNC machine. We specialize in manufacturing CNC routers, laser engraving/cutting machines, plasma cutting machines, laser welding machines and laser marking machines. Our products are widely used in various industries such as arts and crafts processing, advertising, clothing, model- and printed-hectograph making, jewelries, auto parts, packing materials, woodworking and stone working.<br> &nbsp;&nbsp;&nbsp;We have established several branches and agents throughout our country. Our products have been exported to more than 80 countries and regions, such as Korea, the USA, Canada, Australia, Russia, West Europe and Southeast Asia. We have obtained CE and FDA certifications. And each machine will be tested before launching to keep absolute safety. We will offer two-years guarantee for the machines. Because of the advanced management and strict rules of the production, there is zero complaint from our customers, and we will continue to consummate our team.<br> &nbsp;&nbsp;High quality is our eternal pursuit. "Customer first, Credibility first" is our consistent pursuit with supreme faith. We have a complete service network and a top-grade service team. Our service ways and means are Omni-directional, multi-level and high-efficiency, which guarantee the effective and reliable responses to customers in the shortest time.<br> &nbsp;&nbsp;Precision CNC has been providing key technology and pertinent integrated solutions for Industry 4.0 and future factories, helping enterprises to carry out intelligent manufacturing, making intelligent manufacturing to change our work within touch.<br> 3D Cnc Foam Cutting Machine price<br>website:http://www.prxcnc.com/</td></tr></tbody></table>
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by jheny46 at 05-07-2021, 11:40 AM
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<!--StartFragment--><table><tbody><tr><td>Our History<br>Shandong Pucheng Metal Material Co., Ltd. is mainly engaged in stainless steel products business. With continuous development for almost 10 years, the company has established a batch of trusted and reliable suppliers and a stable user group with good quality, variety and complete specifications, and accumulated rich industry knowledge. Looking forward to the future, the company will continue to optimize the industrial structure; continue to apply information technology to enhance the added value of traditional manufacturing, increase the technical content of products, promote the improvement of personnel systems and mechanisms, and improve the overall quality of employees; continue to provide you with products of satisfactory quality and sincere services. The company's business philosophy: "The same product is better than quality, the same quality is better than price, the same price is better than service, and the same service is better than reputation" Professional logistics distribution: professional logistics system guarantees escort for you, fast delivery, timely arrival, and solve your worries! Three guarantees of service: guarantee quality, guarantee time, and guarantee quantity. Service tenet: strong strength, excellent products, low prices and quality service. Solemn promise: guarantee to thank new and old customers for their trust with good products, excellent quality, low price and perfect service. Operating principles: customer first, high quality, complete variety and reasonable price.<br>Our Factory<br>Shandong Pucheng Metal Materials Co., Ltd. is a large-scale stainless steel industry and trade enterprise integrating stainless steel plate wholesale and processing, warehousing and distribution. It is a stainless steel supplier which contains the sales, processing and distribution of stainless steel coil,stainless steel plate and many other services.<br>The company's products cover a variety of Wuxi stainless steel plate series materials, with a standing inventory of 10,000 tons.<br>We always implement the business philosophy and service tenet of "high-speed and efficient, pragmatic development, customer-oriented, service first". With the care and support of colleagues and customers from all over the country, we have continued to develop steadily, and our products have been sold all over the country. The company will serve customers with high-quality products, good reputation and low prices. I would like to express my heartfelt gratitude to the old and new customers who have given us the care and support of our company! And sincerely hope to establish a long-term cooperative relationship with it, mutual benefit and common development!<br>Our Product<br>Main Products: stainless steel plate, stainless steel tube, stainless steel square tube, stainless steel strip, stainless steel coil, stainless steel rod, stainless steel round steel, stainless steel angle steel, stainless steel channel, stainless steel square steel, stainless steel hexagonal steel, stainless steel I-beam, stainless steel flange, stainless steel head, stainless steel valve, valve welding material, wear-resistant steel plate, alloy steel plate, high-strength steel plate, automobile beam plate, container steel plate, pipeline steel plate, decorative materials, various stamping and threaded accessories Various pipe fittings, non-standard custom.<br>Stainless steel material: 431, 301, 303, 304, 321, 316, 316L, 309S, 310S, 405, 410, 420, 420J2, 430<br>Specification: 0.4mm~80mm or customized<br>Product Application<br>Products are widely used in petroleum, metallurgy, food, beverage, electric power, chemical industry, chemical fiber, medical equipment, aviation technical support and consultation, installation and other one-stop services.<br>Our Certificate<br>ISO,BIS,etc<br>Production Equipment<br>Stainless steel tube/carbon steel making machine, round tube polishing machine, square/rectangular tube polishing machine, round tube straightening machine, shaping machine, stainless steel tube, rolling mill, special machine for steel structure processing, special machine for metal sheet processing, slitting Machine, steel (iron) plate/coil, leveling and cutting machine, steel plate processing machine, steel pipe making machine, pipe tapping machine, pipe end trimming machine, pipe cutting machine, pipe bending machine, etc.<br>Production Market<br>Northern Europe, South Asia, Domestic Market, etcSS316 Stainless Steel Angle Bar<br>website:http://www.puchengmaterial.com/</td></tr></tbody></table>
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by jheny46 at 05-07-2021, 11:39 AM
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<!--StartFragment--><table><tbody><tr><td>鈥?Our History<br>We are engaged in Concrete Pump Parts more than 10 years<br>鈥?Our Factory<br>HBYM GROUP is specialized in concrete pump. Business field include: new concrete pump parts and used concrete pump. We have worked in this field more than 10 years. We export goods to Russia, South Korea, America, Canada, Australia, Indonesia, German and India etc. Our aim is superior quality, competitive price and first-class service. We supply spare parts for Putzmeister Pump, Schwing Pump, Zoomlion Pump, CIFA Pump, Kyokuto Pump, etc.<br>鈼?Factory Location: Cangzhou City, Hebei Province, China<br>鈼?Application: PM, Schwing, Sany, Zoomlion, XCMG etc.<br>鈼?Spare parts include: twin wall pipe (DN125*3000mm), twin wall elbow, wear plate and ring,conveying cylinder, rubber piston, forged clamp, S valve, rubber hose etc.<br>鈥?Our Product<br>Concrete pump spare parts: twin wall pipe (DN125*3000mm), twin wall elbow, wear plate and ring, conveying cylinder, rubber piston, forged clamp, S valve etc.<br>鈥?Product Application<br>Putzmeister Pump, Schwing Pump, Zoomlion Pump, CIFA Pump, Kyokuto Pump etc.<br>鈥?Our Exhibition<br>2014 Bauma China Fair, 2016 Bauma China Fair, 2017 EXCON India Fair, 2018 Bauma China Fair, 2019 Bauma Germany Fair.<br>鈥?Production Market<br>Business market is mainly in North America, Eastern Europe, Asia, Mid East, Western Europe and Domestic Market.<br>鈥?Our Service<br>1. Products keep in stock<br>2. Best Quality, Lowest Price<br>3. Support producing as drawing5inch Casting Clamp in stock<br>website:http://www.pumpparts-hbym.com/</td></tr></tbody></table>
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by jheny46 at 05-07-2021, 11:36 AM
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<!--StartFragment--><table><tbody><tr><td>WHO WE ARE <br>COMPANY PROFILE <br>Shenzhen Punair Technology Co, Itd is a national high-tech enterprise in China. The company specializes in the R&amp;D, manufacturing, and marketing functions of the inverter welding &amp; cutting machines. Punair's Vision is to become the world-class supplier of welding&amp;cutting industry while Punair's Mission is to create, develop and contribute to this industrial market all over the world. <br>Punair is located in Chinese First Tier City - Shenzhen which delivers a very convenient transportation network access to the world and shares the border with the international famous metropolis - Hong Kong. <br>The company has expanded its advanced manufacturing facilities to 15,000 square meters in which there serves several mechanized production lines and sufficient sophisticated equipments and devices. <br>By passing the certification requirements of the ISO9001:2000, the company has well developed full ranges of products which have been certified with the CE, CCC, CSA, SAA certificates by the corresponding authorities. <br>"Quality&amp;Innovation Make PUNAIR Standing Out of the World" is our core concept. With this underpinning, Punair is striding on the broaden road to pour its continuous contribution in the technology and market as well as to become one of world-class manufacturing suppliers in the welding&amp;cutting industry. <br>History <br>P series, T series Intelligent, Digitalized and LED Display Products Have Historically Detonated their Demand over the Domestic and Foreign Welding and Cutting Industry Markets;and It Not Only Has Substantially Promoted the Development of Inverter Welding Machines but also Has Made Significant Contributions to the Development of National Industry.<br>Grandly Held the 10th Anniversary Celebration for Punair, Together with the Whole Punair鈥檚 Ecosystem Entities and Relevant Community,Hailing its Slogan of "Gather together, Dream Forward" &amp; Shinning its Core Value of "Inclusive, Perseverant, and Grateful"! <br>Historically Innovated and Forged out the Intelligent, Digitalized and LED Display Brand-new Products, and Grandly Launched into the Global Market. <br>In Great Glory, Won the 4th "Blue Dot Award" of Shenzhen Electronics Chamber of Commerce and the Title of "Pioneer Growth Enterprise" <br>Plunged into New Era of MIG/MAG, and Led to a New Trend of Innovation and Invention of the Industry. Vigorously Increased Investments and Introduced a Fully Automated Advanced SMT Production Line, Which Indicated that the Company Has the Comprehensive Ability to Design, Manufacture and Produce Sophisticated Digital Technology Products. <br>Sales Turnover Hit Record High in the industry, Marking Punair Has Become a Leading Brand in Certain Field of Market segmentation in the Industry. <br>In the Direction of Achieving the Mechanization and Automation of Production, Get Started on the Transformation and Upgrade of the Production and Manufacturing System, &nbsp;&nbsp;Heavily Invested in the Advanced Production Equipment to Build an Advanced Industrial Manufacturing Enterprise. <br>Invented a Product Innovative Development Direction of Integrating Digital Technology and Advanced Welding Technology, and Created the Product Development Model Featuring with Technology Modulization and Product Standardization, Which Enabled Punair to Generate the Integrated Comparative Competitive Advantage Over the Industry. <br>Instituted and Set Multi-brand Strategy as the Cornerstone of Company Development Strategies, Which Is the Right Short-cut of Imprinting Punair Brand鈥檚 Image into Users鈥?Minds, and Gradually Forged Punair and Taylai Brands the High-quality Brands Over the Markets. <br>The Company Won the Honorary Title of "National High-tech Enterprise", Authoritatively Marked Punair鈥檚 Competence and Certified it a Qualified Status and ID of a National High-tech Enterprise. <br>The Global Launch of the Punair New VI for the Company and the Brand, Together with Brand New Product Outlook, Signalled that Punair Has Raised Up as a New Brand Pioneer in the Industry. <br>The Historical Invention of the Smallest 5KG MIG /MAG Welding Machine Denotes that Punair Is a Product Innovator in the Industry. <br>Innovation of Spirit Breaks Through, and Inverter Welding IGBT Technology C Platform Is Invented, which Has Highly Added a Brilliant Shinning to the Development of Technological Innovation in the Industry. <br>Founded in Shenzhen, China. Great Vision Plants in Our Hearts, Great Power Rises from United Team, Great Journey Sets &nbsp;Sail <br>2020 <br>2019 <br>2018 <br>2017 <br>2016 <br>2015 <br>2014 <br>2013 <br>2012 <br>2010 <br>&lt; <br>&gt; <br>SALES NETWORK <br> Excellence is about the team who realizes the most peculiar plans into amazing constructions around us. Throughout years' struggles and endeavour, we have successfully equipped and enriched the world with the best welding equipments to impact customers' business across life while we established a global sales network and built hundreds of strategic partnerships with more than 100 countries, from Norway to Argentina, from the United States to Algeria. You can always find PUNAIR welders on the construction sites or in the garages of the local people. Driven by our core values, we have always been making continuous efforts and development to provide a satisfactory answer to the welding industry of the global market. <br>VISION &amp; MISSION <br> &nbsp;&nbsp;<br> VISION <br>Punair's Vision Is to Become the World-class Supplier of Welding &amp; Cutting Industry. &nbsp;MISSION <br>Punair's Mission Is to Create, Develop and Contribute to the Global Market. &nbsp;CORE CONCEPT <br>Quality&amp;Innovation Make PUNAIR Standing Out of the World. <br> &nbsp;&nbsp;<br> VALUE <br>Employees, customers and industry are our power sources, our value is to keep contributing to the employees, customers and industry throughout our continuous development. &nbsp;BUSINESS PHILOSOPHY <br>Outstanding Quality, Worldwide Punair &nbsp;SLOGAN <br>Punair Welder, Reliable Partner <br>SUSTAINABILLITY <br>Punair highly values ecological and environmental protection. Guided by the conviction that lucid waters and lush mountains are invaluable assets, we advocates harmonious coexistence between humans and nature, and sticks to the path of green and sustainable development. <br>Since inception, on the base of operating in full compliance with applicable laws and regulations, we aspire to achieve the highest business ethics and environmental standards in our cause to become a world-class welding solutions provider as well as an environmental helper. <br>While fulfilling our environmental duties during production, we also carry out many environmental education for the employees to cultivate the protection concepts, and continue to donate and participate in many welfare activities. &nbsp;Industrial Grade TIG DC Inverter TIG 300 manufacturers<br>website:http://www.punairwelder.com/</td></tr></tbody></table>
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by jheny46 at 05-07-2021, 11:34 AM
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<!--StartFragment--><table><tbody><tr><td>Shenzhen Power Tomorrow Actuator Valve Co., Ltd. is a mechanical and electrical integrated high-tech enterprise. <br>We are the largest manufacturer of sprinkler timers in China and a manufacturer of Wireless water spray controllers. Exclusive patent qualification, has obtained CE and ISO9001 international quality certification. In addition to the domestic market, products are also exported to other countries and regions, such as the United States, Europe, Australia, Russia and Hong Kong. <br>With strict and reliable principles and extensive field application experience, we offer the best automation products and solutions in the field of fluid control, such as garden irrigation systems, lawn irrigation systems, indoor plant watering systems. <br>We have a group of enthusiastic, continuously innovative and high-quality R&amp;D staff to forge ahead, and we promise to provide every customers with better products and more intimate service. Of course, it is an endless challenge to create more and better automatic valve. <br>Power-Tomorrow is brave and strong enough to face this challenge. We are sure to achieve this aim. <br> isolation ball valve suppliers<br>website:http://www.qotosmart.com/</td></tr></tbody></table>
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by jheny46 at 05-07-2021, 11:32 AM
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<!--StartFragment--><table><tbody><tr><td>Qinyang City Zhenghua Paper Machinery CO., Ltd is located in Qinyang City, Henan province, China, is a collection of scientific research, design, manufacturing, marketing and service in one of the large-scale papermaking equipment enterprises.<br>The company mainly manufacture all kinds of high-strength corrugated / linerboard machine (used in the production of various types of paper packaging), culture paper machine for the production of printing paper/ A4 paper, toilet paper machine for the production of high-grade household paper, napkins, paper towels etc, special paper making machine include mosquito coil making machine,insole board paper making machine,recycled leather making machine and could supply all kinds of paper making pulping equipment and the spare parts for paper machine.<br>The company has first-class sales and technical service team, according to customer requirements to provide project design, process design and technical training, etc.. We provide a pre-sale, sale, the after-sales tracking service, fully eliminate the worries of the majority of customers.Pre-sale service,supply detailed drawing and technical scheme,provide complete and professional consultation service;after-sale service,send professional installation technician to guide machine installation,and provide technical training for your workers.<br>Our company guarantee 1 year for the paper machine,if any problem of machine,we will recall it and change a new one for free,we ensure supply of spare parts for paper machine.We ensure that our paper machines are of high quality,right mix of quality and price is what we offer to our customer.We have customers from both domestic market and oversea market,main sales market:middle east area,Africa,southeast Asia etc,welcome customers to contact us.Tissue Paper Making Machine<br>website:http://www.qyzhpapermachine.com/<br>website2:http://www.zhpapermachinery.com/</td></tr></tbody></table>
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by jheny46 at 05-07-2021, 11:31 AM
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<!--StartFragment--><table><tbody><tr><td>Dongguan Evergrows Warehouse Storage Equipment Co., Limited is one of the most professional storage racking system manufactory in China. We specialize in research and development, production, sales and installation of various racking systems, including Radio Shuttle Rack, AS/RS Racks(Automated Storage and Retrieval System),Pallet Racks, Shelving Racks, Drive In Racks, Mezzanine Floors, Cantilever Racks, Pallets, Material Handling Containers and more.<br>Our company adopts the finite element analysis software of steel structure rack. The overall strength, stiffness and stability of the rack system are calculated. Our pallet racking's components have been carefully calculated to ensure that they meet the requirements of the FEM10.2.02, FEM9.831, AS 4084-2012.<br>Our factory is located in Dongguan, which is within one hour from Hong Kong, Guangzhou or Shenzhen. Established in 1998, our factory covers an area of around 40,000 square meters, possesses the processing capacity of more than 60,000 tons per year and has more than 20 automatic production lines as well as high-talent management team, professional technicians. We have passed ISO9001:2008 certification in 2004.<br>Due to our good quality, competitive price, fast delivery and upmost customer services, Our products are sold to many countries all over the world. Include United Kingdom, France, Germany, Portugal, Romania, Poland, Turkey, Israel, Saudi Arabia, United Arab Emirates, Bahrain, Qatar, Cyprus, South Africa, Libya, Australia, United States, Canada, Brazil, Uruguay, French Polynesia, Argentina, Chile, Colombia, Philippines, Thailand, Singapore, Indonesia, Hong Kong and more countries and regions.<br>Our professional and hardworking sales are waiting for your inquiries seven days a week from 8 AM to 8 PM. All inquiries, 100% of them, will be replied in the shortest time. OEM and customized service is available upon request. Welcome to visit our factory and establish long term business relationship.<br>Workshop and Factory Facilities:<br>Our HonorTongueallet Racking suppliers<br>website:http://www.rackshelfchina.com/</td></tr></tbody></table>
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by jheny46 at 05-07-2021, 11:29 AM
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<!--StartFragment--><table><tbody><tr><td>Product introduction锛?/strong&gt;<br>Cross bandage design &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br>Adjustable back buckle<br>High impact for exercise<br>Breathable &amp;Skin-friendly<br>Product details锛?/strong&gt;<br>SizeBust(in)Bust(cm)Length(in)Length(cm)<br>S (4-6)26661538<br>M (8-10)27.67015.439<br>L (12-14)29.17415.740<br>XL(16-18)30.77816.141<br>An ever-popular design from Brooks, the Juno model has been updated based on feedback from consumers that its previous iterations were hard to get out of. Now, its redesigned back makes it much more manageable post-run, and its powerful and adjustable Velcro straps are as great as ever. Available up to an E cup. The back USES the I-button design, can stabilize the chest for a long time, reduce the vibration amplitude, avoid discomfort. An ever-popular design from Brooks, the Juno model has been updated based on feedback from consumers that its previous iterations were hard to get out of. Now, its redesigned back makes it much more manageable post-run, and its powerful and adjustable Velcro straps are as great as ever. Available up to an E cup. The bottom circumference can be adjusted, can adjust the appropriate width according to their own bottom circumference.<br>Company Profile<br>We are located in Anhui Province which close to the Shanghai and Ningbo port. In recent years, we have built a reputation as one of China鈥檚 best swimwear manufacturers. We have about 450 skilled veteran workers and stable production lines to make sure the mass production.<br>Our advantage:<br>1.One-stop shop. Once the order placed, the whole Progarments team will make full effort to produce your goods.<br>2.Low MOQ for new starting business. We are willing to grow with you.<br>3.Rich experience for OEM&amp;ODM. Make your own products with own label. We are always here for you.<br>4.Good after-sale service. Track your comments&amp;satisfaction and record them on file for next cooperation and constantly improve our service.custom Women's Active<br>website:http://www.proswimsun.com/women-s-active/</td></tr></tbody></table>
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by FineTest at 04-27-2021, 06:36 PM
<p><b>Abstract</b>:&nbsp; &nbsp;At the beginning of performing monoclonal antibody test, the failure of cell fusion or low fusion rate happens sometimes. Now, some factors for influencing fusion rate are summarized, which may be helpful for experimenters.<br><br><b>Keywords</b>: Monoclonal Antibodies, Fusion Rate, Antibodies Fusion</p><p><br></p><p><b>1. Blood Titer Test</b><br>Before the fusion, the mouse blood should be collected for testing the titer. The titer higher than 20,000 is suitable for the fusion. The fusion effect may not be satisfactory if the titer is lower than 20,000.<br></p><p><br></p><p><b>2. Cell State</b><br>The state of myeloma cells SP2/0 is very important and should be kept without any pollution and large scale death. The logarithmic growth phase of cells should be maintained before the fusion.<br></p><p><br></p><p><b>3. PEG and Fatal Bovine Serum</b><br>The fusion rate depends on the quality of PEG and fatal bovine serum which should be subject to the pilot experiment screening between different brands and batch numbers. Selecting the best quality PEG and fatal bovine serum can ensure the successful rate of fusion efficiently.<br></p><p><br></p><p><b>4. Cell Ratio</b><br>During the fusion, the ratio of SP2/0 and splenocytes should be noticed as well. Although 10:1-1:10 is applicable for the successful fusion, the best fusion efficiency is between 2:1-1:2. No matter which ratio, the two kinds of cells don't contain serum components and should be mixed well. During the fusion process, the fusion tube should be always rotated. Dropping PEG and terminating the fusion should be slowly first and fast afterwards to avoid the great impact on the cell.<br></p><p><br></p><p><b>5. Feeder Layer Cells</b><br>Feeder layer cells can also influence the fusion effect. Feeder cells of hybridoma can be chosen from mouse peritoneal macrophages, splenocytes and thymocytes. The peritoneal macrophages is the best choice. Meanwhile, the amount of feeder cells in each well should be also noticed. Excessive cells will fight for medium nutrition, making the growth of hybridoma cells difficult to maintain. Deficient cells can't serve as the feeder layer. Hence, only the reasonable cell amount can achieve the good effect.<br></p><p><br></p><p><b>6. Medium Additives</b><br>The intelligent use of some additives has a good prospect. E.g. the hybridoma cell culture medium additive developed by FineTest can efficiently extend the service life of medium, improve the growth speed of hybridoma cells and increase fusion rate. Under unsatisfactory experimental conditions, the fusion rate can be still maintained well.<br></p><p><br></p><p><img src="https://bit.ly/3vk5FEc"><br></p><p><b>Figure 1.</b>&nbsp; Hybridoma Cell Culture Medium Additive(100X)<br></p><p><br></p><p><img src="https://bit.ly/3eAzYj3"><br></p><p><b>Figure 2.</b> Comparison between fusion cells without medium additives and hybridoma cells with medium additives.<br></p><p><br></p><p><b>7. Conclusion</b><br>There are other factors for influencing fusion rate. E.g.: the number of cells in each well after the fusion, centrifugal rotational speed and time, the strength for pipetting cells etc. During the actual experimental process, it's important to accumulate more experience.<br></p>
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