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by natarajan at 06-07-2019, 03:39 AM
I would like to know the compiled list of all branches of Mtech / MS  offered by the IITs and other premier institutes in India for which one can apply through valid score in GATE biotechnology etc. I want to know this to get an idea of what other branches one can apply for in addition to biological sciences with a valid score in GATE BT. Kindly let me know.Thanking in advance.
by SuperLabTech at 05-11-2019, 01:48 AM
[Image: source.gif]

29-year-old lab technician from New Jersey, USA. I have been meaning to join a forum like this about biotechnology. I'm looking forward to checking the place out.
by AlexGoldman at 03-30-2019, 01:21 AM
Hello! I am interested in gene engineering technologies, but for now my knowledge is not so large.
Some days ago i was watching TED show about CRISPR technology(this one, and there was an information about editing of the full organism (means editing genome of each cell) using viruses.
I had an idea about it yet, but I actually don't know how does it work. How can we use viruses to change cell's genes. Could you please tell about it or give some information or books about that theme? Thank's a lot!

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by Levi4than at 03-04-2019, 08:30 AM
Is it possible to embed transmembrane domain to the protein by inserting sequence which codes mentioned domain to gene that codes the enzyme, in the way that it would allow the protein to become an IMP (integral membrane protein) without depriving it of its enzymatic properties?

I would really appreciate a helpful answer.
by Sushmitha at 02-27-2019, 04:01 AM
Can anybody please suggest me some good seminar topics for biotechnology engineering

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by Camomile at 02-20-2019, 05:31 AM
Hi Everyone!
I am really pleased to join your community. I find it really enriching and useful.

Today I am writing to you with a really broad question regarding background referred to analytical chemistry.
Currently, I am writing my Master thesis and due to the fact that am only an engineer of biotech; some from aspects of pure analytical chemistry is really not understandable.

Honestly, I am left without an advisor and have nobody to ask for help.
Lets move to the point; I am ordering my results and found myself in a position where I do not understand the difference in the application of cyclic voltammetry and square wave voltammetry.

I made few measurements of a suspension containing an enzyme and a buffer ( not activity one ). I hold in a hand a publication where research group applied a square wave voltammetry and as a standardized value; applied a specific pick on exact potential. I will be really grateful for receiving a short explanation about the difference between those two techniques.
by Gunaseelan at 11-26-2018, 03:31 PM
Hello, everyone.i am gunaseelan ,2nd year btech biotech many professionals guides biotech students .I am really happy to join this community.thanks everyone...

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by NikitaM at 11-03-2018, 11:13 PM
Hello dude,

Good evening to all. I'm a newbie here. I would like to introduce myself with all of you guys. I hope everyone here is doing well so far.

Thank you so much!
by sj26 at 10-24-2018, 04:30 AM
From where i can learn perl programming for bioinformatics??
by SACHIN THOMAS at 10-03-2018, 05:33 AM
Hello all,
I'm a student doing my bachelor's in Biotechnology Engineering.
Hope I can contribute and learn more from this platform.
Thank You
by sonali yegure at 09-30-2018, 12:05 PM
Hiii i am a new in this forum......
Plz help me to know a recent Biotechnological researches and best project to do in UG level....
by bhavana k v at 09-17-2018, 01:03 PM
I am a student of MSc biotechnology in Bangalore please tell me the project topics which can be finished in 2.5months and which companies offer project for students .
by Kautilya Srivastava at 09-17-2018, 01:01 AM
Can anyone suggest about any summer internship for biotechnology around the world?
by Sonasrikanth at 09-16-2018, 01:12 PM
How to get a job in biotechnology field?
by RadhikaPophali at 09-08-2018, 03:54 AM
Biotechnology is the application of technologies to the conventional biology with a goal of betterment of human life. The scope of biotechnology is vast and understanding the basics of biotechnology has become essential for the life science students. One of the important areas of biotechnology is recombinant DNA technology. This technology is the essence of many useful strategies widely used in healthcare, medicine, and nutrition. This article gives the basic idea about the topic and discusses its scope.
Recombinant DNA is the DNA which is formed by the combination of DNA from two or more sources. While studying the basics of this technology, one must understand some important terms and students should know about:
·        Genome: The genetic constitution of an organism
·        Clones: Genetically identical organisms
·        Cloning: Introduction of foreign DNA into the host cell
·        Vector: Carrier of the gene of interest (ex. Plasmid, Phage)
·        Marker: Gene for the identification of transformant cells
·        Insert: Gene of interest carried by the vector to the host cell
·      Recombinant DNA: DNA formed by joining the DNA from different origins, mostly used for a combination of vector and insert
·        Host: Recipient cell for foreign DNA
·        Copy number: Copy number of a vector denotes the number of copies it can produce within a cell
Tools used for recombinant DNA technology are the molecules, mainly enzymes, used for the manipulation of DNA like cutting, joining, end modification etc. (1). Some are enlisted below.
·        Restriction enzymes:  Popularly known as “molecular scissors”
·        Ligase: Popularly known as “molecular glue”
·        Topoisomerase
·        End modification enzymes: Polynucleotide kinases, nucleases
·        Linkers, adapters, and connectors.
The central dogma of recombinant DNA technology
A.  Insert selection: Insert is typically the gene of interest which we desire to clone
B.   The formation of a recombinant DNA: It involves the use of restriction enzymes as well as a ligase. It is the joining of an insert to a vector.
C.   Cloning of this recombinant DNA into a host cell.
D. Expression of the gene of interest
The whole aim for gene transfer exercises is either
A.  Phenotypic expression of a foreign gene in an organism as can be seen in case of crop improvement techniques
B.   Production of a particular protein in bulk
Ex. Heterologous protein production like the production of human insulin in bacteria
This technology is widely used in healthcare industries, agriculture, clinical research etc. Apart from this, it is also used for gene therapies including various genetic disease.
While using this technology, expressing a foreign gene in a given host is not that easy. Obviously, the host cell has to make a lot of compromises. Research is going on to address this issue (2). Recombinant DNA technology is further being expanded as synthetic biology (3), which will be the main driving force for therapeutics, food and energy sector and a lot more industries.
  2. Ceroni F, Algar R, Stan G, Ellis T et al (2018) Nature Methods, 15:387–393,
  3. Katz L, Chen YY, Gonzalez R et al (2018) J Ind Microbiol Biotechnol, 45: 449.
by kamsharma at 09-02-2018, 04:22 AM
Plzzz tell me abt energy rich metabolites
by akshat28 at 09-01-2018, 05:09 PM
I m doing b tech biotechnology what are online courses I can apply for
by Jobkeeper at 08-27-2018, 12:26 AM
Hi guys, how can one storage genetic material such as DNA at home for several years? 
Also, what requirements should there be to the freezer that is used, for example should the 
freezer be able to freeze at -20? 

Thank you for answering.
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