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by Serbin231 at 01-08-2020, 08:54 AM
Is there any user friendly way to find rare mutations in the individual human whole genome sequencing raw data? (from Dante, 30x coverage).

To be more specific, I want to find mutations from this paper: https://docs.google.com/document/d/1EkRM...sp=sharing (it's very short, less than one page).

But I'm confused. In their paper they just talk about genes (RAD21, B3GAT2, SMC3, SCN11A , SCN5A, SCN9A, SCN10A, SCN11A, TRPA1), but not mutations? Can we find thats diseased-mutations which they talking about in genes, which their list? Or is there not enough data in the paper/study for this?

And if there is not enough data, which data I need to request from authors?

Or those genes have kind a "gold standard sequences" and if that sequence different from standard - there is "diseased" gene? (I have only very basic genetics and bioinformatics knowledges)
by CHANDA7 at 09-16-2019, 12:54 AM
can anyone help me by defining what is "minimal promoter" and "basal promoter"? what are the elements they consist of? what are the basic difference between the two
by nraj at 07-27-2019, 01:36 AM
Hi All,

I recently started working in a biotech incubator offering space and equipment to about 5 start ups.I just wanted to cross reference some info I have been given. Can someone please offer me a rundown of the most commonly used biotech lab equipment necessary for an ordinary start up. And can you please segment that list in this way.

1) Lab Equipment that can be shared between companies

2) Lab Equipment that cannot be shared between companies

3) Lab Equipment that isn't completely necessary, but would be helpful to have in a lab space.

I hope you all can help me out. Thanks!
by priyansha sinha at 07-11-2019, 06:29 AM
Dear sir,

I've completed my B.Tech degree in biotechnology this year and qualified GATE as well. Since I've not taken admission in any college, I am looking to work as an intern in some company or to do a project in R&D department (Biotech) in any institute. I kindly request you to help me and let me know if there's any such opportunity available in India (specifically Bangalore) that I could pursue.

Thank you
Priyansha
by YashulBhardwaj at 07-10-2019, 08:09 AM
I Yashul Bhardwaj from haryana, i recently qualify the JNU-CEEB 2019 for M.tech Biotech with rank of 15.
It is basically a DBT Sponsored Program with the fellowship equivalent to GATE.
After seat allotment, i got allotment at West Bengal University of Technology (Kolkata).

My Request is that, is this alloted university is good enough to pursue the M.tech as the N.I.Ts are in terms of the hands on training and placement ?? Because I am not getting much info about this from net surfing.

Kindly suggest me, should I go for M.tech in this university or not??  ... as the seat blocking procedure is about to begin from 11th to 14th July.
Kindly response on this as soon as possible.
by natarajan at 06-07-2019, 03:39 AM
I would like to know the compiled list of all branches of Mtech / MS  offered by the IITs and other premier institutes in India for which one can apply through valid score in GATE biotechnology etc. I want to know this to get an idea of what other branches one can apply for in addition to biological sciences with a valid score in GATE BT. Kindly let me know.Thanking in advance.
by SuperLabTech at 05-11-2019, 01:48 AM
[Image: source.gif]

29-year-old lab technician from New Jersey, USA. I have been meaning to join a forum like this about biotechnology. I'm looking forward to checking the place out.
by Levi4than at 03-04-2019, 08:30 AM
Is it possible to embed transmembrane domain to the protein by inserting sequence which codes mentioned domain to gene that codes the enzyme, in the way that it would allow the protein to become an IMP (integral membrane protein) without depriving it of its enzymatic properties?

I would really appreciate a helpful answer.
by Sushmitha at 02-27-2019, 04:01 AM
Can anybody please suggest me some good seminar topics for biotechnology engineering

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by Camomile at 02-20-2019, 05:31 AM
Hi Everyone!
I am really pleased to join your community. I find it really enriching and useful.

Today I am writing to you with a really broad question regarding background referred to analytical chemistry.
Currently, I am writing my Master thesis and due to the fact that am only an engineer of biotech; some from aspects of pure analytical chemistry is really not understandable.

Honestly, I am left without an advisor and have nobody to ask for help.
Lets move to the point; I am ordering my results and found myself in a position where I do not understand the difference in the application of cyclic voltammetry and square wave voltammetry.

I made few measurements of a suspension containing an enzyme and a buffer ( not activity one ). I hold in a hand a publication where research group applied a square wave voltammetry and as a standardized value; applied a specific pick on exact potential. I will be really grateful for receiving a short explanation about the difference between those two techniques.
by Gunaseelan at 11-26-2018, 03:31 PM
Hello, everyone.i am gunaseelan ,2nd year btech biotech student.here many professionals guides biotech students .I am really happy to join this community.thanks everyone...

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by NikitaM at 11-03-2018, 11:13 PM
Hello dude,

Good evening to all. I'm a newbie here. I would like to introduce myself with all of you guys. I hope everyone here is doing well so far.

Thank you so much!
by sj26 at 10-24-2018, 04:30 AM
Hello
From where i can learn perl programming for bioinformatics??
by SACHIN THOMAS at 10-03-2018, 05:33 AM
Hello all,
I'm a student doing my bachelor's in Biotechnology Engineering.
Hope I can contribute and learn more from this platform.
Thank You
by sonali yegure at 09-30-2018, 12:05 PM
Hiii i am a new in this forum......
Plz help me to know a recent Biotechnological researches and best project to do in UG level....
by bhavana k v at 09-17-2018, 01:03 PM
I am a student of MSc biotechnology in Bangalore please tell me the project topics which can be finished in 2.5months and which companies offer project for students .
by Kautilya Srivastava at 09-17-2018, 01:01 AM
Can anyone suggest about any summer internship for biotechnology around the world?
by Sonasrikanth at 09-16-2018, 01:12 PM
How to get a job in biotechnology field?
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