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by SACHIN THOMAS at 10-03-2018, 05:33 AM
Hello all,
I'm a student doing my bachelor's in Biotechnology Engineering.
Hope I can contribute and learn more from this platform.
Thank You
by sonali yegure at 09-30-2018, 12:05 PM
Hiii i am a new in this forum......
Plz help me to know a recent Biotechnological researches and best project to do in UG level....
by bhavana k v at 09-17-2018, 01:03 PM
I am a student of MSc biotechnology in Bangalore please tell me the project topics which can be finished in 2.5months and which companies offer project for students .
by Kautilya Srivastava at 09-17-2018, 01:01 AM
Can anyone suggest about any summer internship for biotechnology around the world?
by Sonasrikanth at 09-16-2018, 01:12 PM
How to get a job in biotechnology field?
by RadhikaPophali at 09-08-2018, 03:54 AM
RECOMBINANT DNA TECHNOLOGY: OVERVIEW AND SCOPE
 
SUMMARY
Biotechnology is the application of technologies to the conventional biology with a goal of betterment of human life. The scope of biotechnology is vast and understanding the basics of biotechnology has become essential for the life science students. One of the important areas of biotechnology is recombinant DNA technology. This technology is the essence of many useful strategies widely used in healthcare, medicine, and nutrition. This article gives the basic idea about the topic and discusses its scope.
 
BASICS
Recombinant DNA is the DNA which is formed by the combination of DNA from two or more sources. While studying the basics of this technology, one must understand some important terms and students should know about:
·        Genome: The genetic constitution of an organism
·        Clones: Genetically identical organisms
·        Cloning: Introduction of foreign DNA into the host cell
·        Vector: Carrier of the gene of interest (ex. Plasmid, Phage)
·        Marker: Gene for the identification of transformant cells
·        Insert: Gene of interest carried by the vector to the host cell
·      Recombinant DNA: DNA formed by joining the DNA from different origins, mostly used for a combination of vector and insert
·        Host: Recipient cell for foreign DNA
·        Copy number: Copy number of a vector denotes the number of copies it can produce within a cell
 
Tools used for recombinant DNA technology are the molecules, mainly enzymes, used for the manipulation of DNA like cutting, joining, end modification etc. (1). Some are enlisted below.
 
·        Restriction enzymes:  Popularly known as “molecular scissors”
·        Ligase: Popularly known as “molecular glue”
·        Topoisomerase
·        End modification enzymes: Polynucleotide kinases, nucleases
·        Linkers, adapters, and connectors.
 
The central dogma of recombinant DNA technology
A.  Insert selection: Insert is typically the gene of interest which we desire to clone
B.   The formation of a recombinant DNA: It involves the use of restriction enzymes as well as a ligase. It is the joining of an insert to a vector.
C.   Cloning of this recombinant DNA into a host cell.
D. Expression of the gene of interest
 
 
APPLICATIONS
The whole aim for gene transfer exercises is either
A.  Phenotypic expression of a foreign gene in an organism as can be seen in case of crop improvement techniques
B.   Production of a particular protein in bulk
Ex. Heterologous protein production like the production of human insulin in bacteria
 
This technology is widely used in healthcare industries, agriculture, clinical research etc. Apart from this, it is also used for gene therapies including various genetic disease.
 
FUTURE
While using this technology, expressing a foreign gene in a given host is not that easy. Obviously, the host cell has to make a lot of compromises. Research is going on to address this issue (2). Recombinant DNA technology is further being expanded as synthetic biology (3), which will be the main driving force for therapeutics, food and energy sector and a lot more industries.
 
REFERENCES
  1. https://link.springer.com/chapter/10.100...7-2460-8_3
  2. Ceroni F, Algar R, Stan G, Ellis T et al (2018) Nature Methods, 15:387–393, https://doi.org/10.1038/nmeth.4635
  3. Katz L, Chen YY, Gonzalez R et al (2018) J Ind Microbiol Biotechnol, 45: 449. https://doi.org/10.1007/s10295-018-2056-y
by kamsharma at 09-02-2018, 04:22 AM
Plzzz tell me abt energy rich metabolites
by akshat28 at 09-01-2018, 05:09 PM
I m doing b tech biotechnology what are online courses I can apply for
by Jobkeeper at 08-27-2018, 12:26 AM
Hi guys, how can one storage genetic material such as DNA at home for several years? 
Also, what requirements should there be to the freezer that is used, for example should the 
freezer be able to freeze at -20? 

Thank you for answering.
by Matthewdlr4 at 08-21-2018, 08:06 AM
Hello, I'm posting here in hopes someone on this forum can point me in the right direction.
I'm interested in genetically modifying avian species such as chickens. I am trying to make a breed of chicken that can have the attribute of a homing pigeon that allows it to find its way home.
Does anyone have any books, threads, blogs, articles that someone with little knowledge about this topic can understand? Any help would be appreciated
by ankbajaj000@gmail.com at 08-20-2018, 01:16 AM
What is the scope and best institutes for pursuing msc in biotechnology if a student has PCB in class 12?
by nikki at 08-20-2018, 12:41 AM
I want to go for an offline coaching in pune for gate.Are there any please ppl let me know fast ?
by Preeti Sharma at 08-14-2018, 05:41 AM
Hi Sir/Ma'am,

I am fresher, I have completed my B. Sc +M.Sc in biotechnology in may 2018 only. I am not preparing for CSIR right now. I need a job related to my field only as my core interest is in R&D only. I have good internship records. Please suggest me some jobs suitable to my profile. I am very interested in Forensic DNA Analyst profile
.
With Regards.
by Fazlil at 08-14-2018, 03:23 AM
Can anyone send me the soft copy of "Bioinformatics- Methods and Applications: Genomics, Proteomics and Drug Discovery"(4th edition) by S.C. Rastogi, P. Rastogi, N. Mendiratta
by mpancharia@gmail.com at 08-09-2018, 12:25 AM
I want to do my career in Regenerative medicine, abroad (primarily US or Germany/ Netherlands)

I am presently pursuing Medical Biotechnology from DY Patil University, Pune in my final year.

I wish to have a better chance of good job overseas and for that, I do wish to do my masters there itself (abroad).

Now my query, as a need of my curriculum, I am supposed to do a 6-month internship during my final semester (i.e from Jan 2019 to June 2019) - I want to use this opportunity of Internship for getting me the best possible career in Regenerative Medicine field.

Hence, want suggestions of all the stalwarts as well as members here, on which place (private lab or university) must I do my Internship - I am also ready to go abroad for this internship, to boost my career - and also wish that this internship should help in landing me into the best possible University for Regenerative medicine.

I hope i am able to pen down my query well.  Your response is highly appreciated.

Many thanks to all of you here well in advance. Smile
by Arie Helderman at 08-08-2018, 07:16 PM
Events: SIG Workshop to discuss developments in computational pathology and its interfacing opportunities with bioinformatics during ECCB 2018

Sunday, September 9, 2018
Athens, Greece (during ECCB2018)
cpw2018.org

Submission deadline for abstracts has been extended until August 15. Travel awards available!

Digital and computational pathology are increasingly used to study biological processes and diseases as novel molecular probing and imaging techniques allow the measurement of single molecules in whole tissue sections. Resulting multi-gigapixel images can be viewed on a computer screen via dedicated software. However, automated analysis of such large-scale datasets is challenging and their combination with omics data is not trivial. This workshop wants to facilitate bridging opportunities between the bioinformatics and tissue image analysis communities.

This second edition is organized as a full-day workshop during ECCB 2018 (www.eccb18.org).
SCOPE
Computational pathology exists at the interphase between bioinformatics, machine learning, and digital pathology (microscopy). Submissions from all topics of interest are welcome (non-exhaustive list):

Machine learning and image analysis for quantification in digital pathology (in 2D & 3D), Novel approaches (e.g. spatial or graph-based methods) to extract high-level features (e.g. molecular networks or spatial expression patterns) from tissue images for systems pathology, Software development methodologies and tools to ease the exploitation of large tissue images and integration with omics data, Novel molecular imaging techniques on tissues (molecular pathology, next generation pathology) and their analysis challenges, e.g. in situ hybridization, mass spectrometry imaging of formalin-fixed paraffin-embedded tissues, mass cytometry, and other spectroscopy techniques (Raman, ...), ... Research applications on non-human model organisms
KEYNOTE SPEAKERS
The following keynote speakers are confirmed to speak at the conference:

Thomas Fuchs, Memorial Sloan Kettering Cancer Center
Chakra Chennubhotla, University of Pittsburgh
Derek Magee, University of Leeds
SUBMISSIONS
The conference solicits oral talks, posters, and proceeding papers contributions of original work or work that was recently accepted or published in a peer-reviewed journal or at a high level international conference. In the latter case, the publication reference should be clearly mentioned, and the abstracts should be checked mainly for relevance, rather than receive a full review.

Conference proceedings will be published by the Journal of Pathology Informatics.

The program committee will decide which contributions are selected for an oral presentation, and which ones are presented during a poster session. For submission instructions, please refer to www.fourwav.es/cpw2018
KEY DATES
Submission deadline: August 15, 2018.
Conference: Sunday September 9, 2018.
by jackhenry at 08-07-2018, 06:26 PM
want to know about IHC Protocol? Guide me.
by brindhaa at 08-02-2018, 02:09 AM
Hi, i am a b.tech biotech student. Can anyone say some companies and their details for internships known to u in the surroudings of chennai and bangalore..??
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