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Acid for TMB in Elisa Kits
What is the acid in the stop solution for TMB in ELISA kits? Can anyone tell me?
Studying Biotechnology from Merced College in California.
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Hey Jones, I think what you mean is the sulfuric or phosphoric acid stop solution. Here’s a link I found:
Lyka Candelario, RN
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Stopping reagent for peroxidase and TMB: 3 M HCl or 3 M H2SO4

you need to use 50ul for total 450ul reaction. so final concentration will be ~0.33 M. Final concentration can vary from 0.2M to 1M.
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