07-17-2013, 08:57 PM
08-21-2013, 12:50 AM
Hey Jones, I think what you mean is the sulfuric or phosphoric acid stop solution. Here’s a link I found:
http://www.piercenet.com/browse.cfm?fldI...5D985DFDEA
http://www.piercenet.com/browse.cfm?fldI...5D985DFDEA
12-31-2013, 09:15 PM
Hello
Stopping reagent for peroxidase and TMB: 3 M HCl or 3 M H2SO4
you need to use 50ul for total 450ul reaction. so final concentration will be ~0.33 M. Final concentration can vary from 0.2M to 1M.
Stopping reagent for peroxidase and TMB: 3 M HCl or 3 M H2SO4
you need to use 50ul for total 450ul reaction. so final concentration will be ~0.33 M. Final concentration can vary from 0.2M to 1M.