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+--- Thread: Acid for TMB in Elisa Kits (/thread-2362.html)
Acid for TMB in Elisa Kits - JonesMiller - 07-17-2013
What is the acid in the stop solution for TMB in ELISA kits? Can anyone tell me?
RE: Acid for TMB in Elisa Kits - lyka_candelario - 08-21-2013
Hey Jones, I think what you mean is the sulfuric or phosphoric acid stop solution. Here’s a link I found:
http://www.piercenet.com/browse.cfm?fldID=1B18214D-502A-4B8A-AC2E-D75D985DFDEA
RE: Acid for TMB in Elisa Kits - brijnbhatt - 12-31-2013
Hello
Stopping reagent for peroxidase and TMB: 3 M HCl or 3 M H2SO4
you need to use 50ul for total 450ul reaction. so final concentration will be ~0.33 M. Final concentration can vary from 0.2M to 1M.