[Mandokir]

Thank you for your answer,

However i belive that conventional DNA amplification methods are unsuitable for our research.

The single cells are trapped inside a cilinder shaped container with a vollume of 1.5nl. We cannot remove the cells, we can only add reagents. However when we attempt to lyse the cells DNA will be lost if it cannot be bound to something, so we add DNA binding beads.

Next we want to perform a DNA amplification, the problem is that we cannot remove the beads efficiently either. Thus we want to perform the DNA amplification inside the 1.5nl cilinders. We cannot perform a PCR due to isuues with the carrier material. Thus we need to perform a non PCR amplification.

So we are bound to the folowing restrictions:

- We cannot remove the genomic DNA bound to the beads
- We cannot perform PCR
- We cannot perform anny emulsion techniques
- The amplification has to be performed in a 1.5nl vollume
- We need to amplify the whole genome

Thus: can we amplify whole genomic DNA bound to beads in anny way with the above restrictions aplied?

What would happen if we just add amplification reagents to the DNA bound to the beads? Would the DNA be amplified? Would the formed DNA also stick to the beads?

Greetings!