EcoRI restriction sites on 10Kb DNA is shown.
Upon partial digestion, what are lengths of all possible DNA fragments obtained (in kb)? 

(02-16-2017, 01:33 AM)Manoj_Shastry Wrote: EcoRI restriction sites on 10Kb DNA is shown.
Upon partial digestion, what are lengths of all possible DNA fragments obtained (in kb)? 

I choose option 1

(02-16-2017, 01:48 AM)I guess the answer is "C".  Not to forget that its a DNA feagment and not a circular plasmid so 5 Kb frgmanet is not possible. I might be wrong please correct me if I am. CHANDA7 Wrote:

(02-16-2017, 01:33 AM)Manoj_Shastry Wrote: EcoRI restriction sites on 10Kb DNA is shown.
Upon partial digestion, what are lengths of all possible DNA fragments obtained (in kb)? 

I choose option 1

(02-16-2017, 03:10 AM)I am sorry. Option A is the right answer :) pragari Wrote:

(02-16-2017, 01:48 AM)I guess the answer is "C".  Not to forget that its a DNA feagment and not a circular plasmid so 5 Kb frgmanet is not possible. I might be wrong please correct me if I am. CHANDA7 Wrote:

(02-16-2017, 01:33 AM)Manoj_Shastry Wrote: EcoRI restriction sites on 10Kb DNA is shown.
Upon partial digestion, what are lengths of all possible DNA fragments obtained (in kb)? 

I choose option 1

Can anyone please answer this?

(02-15-2017, 05:36 PM)Akshay shinde Wrote:

(02-15-2017, 04:00 AM)RONIT SHARMA Wrote:

(02-15-2017, 03:59 AM)Akshay shinde Wrote: Which one of the following graphs represents kinetics of protein precipitation...

"A"

Sunil sir, what is your opinion on this question ?

I guess the confusion is at the word "free protein"... assuming that by free protein one refers to precipitated protein, then option C is correct. Otherwise, as a classical case, option A is right.

Moreover, are they expecting an assumption of progressive addition of ammonium sulphate? (that's another point to ponder)...

hello sunil sir,
another question

techniques used to determine the structure of a 15KDa globular protein at atomic resolution
1.raman
2.ir
3.UV
4.NMR

Q: A bacteria has genome of size 6 million bp. If DNA synthesis rate is 1000 bp/ second. How long does it take (in minutes) for replication of genome?

Ans: Size= 6000000bp
Speed= 1000bp/second
Time needed= 6000000/1000 seconds = 6000 seconds = 100 mins


Here the assumption is that there is only one replication fork.

Keep a note in future that, each replication fork works independently at the same pace. Therefore, had there been 2 replication forks, time taken would have been 100/2 mins I.e 50mins . 33.33 mins for 3 forks and 25mins for 4 forks and so on..

gate 2017 numerical

a DNA strand of length 25nm wraps diametrically around the circumferance of a spherical histone octamer once.the radius of the histone-octamer is

Q: A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Which technique can verify gene duplication?
A. Northern blotting
B. Southern blotting
C. South western blotting
D. Western blotting

Southern blotting to detect gene duplicates