01-07-2014, 03:36 AM
Green fluorescent protein and its principles have thrown their light over the wide spectrum of biological causes. Today, with the help of color palette which is comprised of fluorescent proteins in various spectrum allow researchers to paint living cells according to their own desires.
Besides, sophisticated bio sensors have been engineered with regard to containing single or double or multiple fluorescent proteins. These include spatiotemporally FRET-based bio sensors. They unveil molecular methodologies involved in underlying physiological processes.
It is also true that these molecular have immensely contributed for basic researches so far. It is also necessary to mention that their functionality could be used in applied sciences is yet to investigate.
Genetically produced bio sensors has the tendency to allow noninvasive imaging of certain bio recognition or biochemical processes with the preservation of temporal and sub-cellular spatial information. Apart from this, aequorea green fluorescent protein and their engineered variants are very important and critical components of genetically produced bio sensors because they provide read-out of bio-recognition chain of events even during investigations.
The family of fluorescent protein based bio sensors includes the diverse range of arrays of various designs that can be utilized for different photo-physical characteristics of fluorescent proteins.
Notwithstanding, these fluorescent bio sensors can be used for studying molecular chain of events from single cells to whole organism. They are unique in the context that they have the capability to target both organelles and tissues. They can also determine different molecules events such as protein binding interactions, phosphorylation, proteolysis, and De-phosphorylations. There is option of designing them with four different and common patterns.
These patterns includes intra-molecular interactions, intermolecular interactions, multiple FPs or proteo-lytic cleavage. Binding interactions between proteins are essential for signal transduction and catalytic activation. For instance, many protein pathways are activated by the association of membrane receptors, which activate enzymes for propagation of signals.
Besides, sophisticated bio sensors have been engineered with regard to containing single or double or multiple fluorescent proteins. These include spatiotemporally FRET-based bio sensors. They unveil molecular methodologies involved in underlying physiological processes.
It is also true that these molecular have immensely contributed for basic researches so far. It is also necessary to mention that their functionality could be used in applied sciences is yet to investigate.
Genetically produced bio sensors has the tendency to allow noninvasive imaging of certain bio recognition or biochemical processes with the preservation of temporal and sub-cellular spatial information. Apart from this, aequorea green fluorescent protein and their engineered variants are very important and critical components of genetically produced bio sensors because they provide read-out of bio-recognition chain of events even during investigations.
The family of fluorescent protein based bio sensors includes the diverse range of arrays of various designs that can be utilized for different photo-physical characteristics of fluorescent proteins.
Notwithstanding, these fluorescent bio sensors can be used for studying molecular chain of events from single cells to whole organism. They are unique in the context that they have the capability to target both organelles and tissues. They can also determine different molecules events such as protein binding interactions, phosphorylation, proteolysis, and De-phosphorylations. There is option of designing them with four different and common patterns.
These patterns includes intra-molecular interactions, intermolecular interactions, multiple FPs or proteo-lytic cleavage. Binding interactions between proteins are essential for signal transduction and catalytic activation. For instance, many protein pathways are activated by the association of membrane receptors, which activate enzymes for propagation of signals.
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