09-26-2013, 09:25 PM
Extremophiles and detection techniques
Modern biotechnology techniques and biochemical detection methods including restriction fragment length polymorphism (RFLP), polymerase chain reaction (PCR), rRNA sequence analysis and sensitive colorimetric methods are improving bacterial detection and enabling better distinction of bacterial species.
Extremophiles are organisms that withstand and thrive in extreme conditions such as extreme heat or highly acidic conditions. Most extremophiles are microbes. An example would be the Thermus aquaticus bacteria which survive in hot springs and from which Taq polymerase is extracted for polymerase chain reaction (PCR). Another extremophile is the microorganism HSL10. This was identified as a member of the genus Microbacterium by morphological observation, Gram staining and sequence analysis of the 16S rRNA gene and the 16S-23S rRNA intergenic spacer region Microbacteria are a genus of bacteria in the family Microbacteriaceae, which have been isolated from both environmental and clinical sources. Like other extremophiles, HSL10 has been identified as a potential source of enzymes such as alkaline protease. It has been confirmed to be capable of secreting alkaline proteases; this capacity was increased by both NaCl and yeast extract. In order to sensitively detect protease production by HSL10 under neutral and alkaline conditions, a sensitive colorimetric method was developed by using the classical Bradford reagent for substrate staining to improve the contrast between the hydrolyzation zone and the substrate background on agar plates. The isolation and characterization of HSL10 has both academic and commercial importance and viable detection techniques play an important role in exploiting this and other microorganisms.
Sources
FUNKE, G., FALSEN, E. and BARREAU, C., 1995. Primary identification of Microbacterium spp. encountered in clinical specimens as CDC Coryneform Group A-4 and A-5 Bacteria. Journal of Clinical Microbiology, 33(1), pp. 188–192
LÜ, J. et al., 2013. An extremophile Microbacterium strain and its protease production under alkaline conditions. Journal of Basic Microbiology; Date of Electronic Publication: 2013 May 17
Modern biotechnology techniques and biochemical detection methods including restriction fragment length polymorphism (RFLP), polymerase chain reaction (PCR), rRNA sequence analysis and sensitive colorimetric methods are improving bacterial detection and enabling better distinction of bacterial species.
Extremophiles are organisms that withstand and thrive in extreme conditions such as extreme heat or highly acidic conditions. Most extremophiles are microbes. An example would be the Thermus aquaticus bacteria which survive in hot springs and from which Taq polymerase is extracted for polymerase chain reaction (PCR). Another extremophile is the microorganism HSL10. This was identified as a member of the genus Microbacterium by morphological observation, Gram staining and sequence analysis of the 16S rRNA gene and the 16S-23S rRNA intergenic spacer region Microbacteria are a genus of bacteria in the family Microbacteriaceae, which have been isolated from both environmental and clinical sources. Like other extremophiles, HSL10 has been identified as a potential source of enzymes such as alkaline protease. It has been confirmed to be capable of secreting alkaline proteases; this capacity was increased by both NaCl and yeast extract. In order to sensitively detect protease production by HSL10 under neutral and alkaline conditions, a sensitive colorimetric method was developed by using the classical Bradford reagent for substrate staining to improve the contrast between the hydrolyzation zone and the substrate background on agar plates. The isolation and characterization of HSL10 has both academic and commercial importance and viable detection techniques play an important role in exploiting this and other microorganisms.
Sources
FUNKE, G., FALSEN, E. and BARREAU, C., 1995. Primary identification of Microbacterium spp. encountered in clinical specimens as CDC Coryneform Group A-4 and A-5 Bacteria. Journal of Clinical Microbiology, 33(1), pp. 188–192
LÜ, J. et al., 2013. An extremophile Microbacterium strain and its protease production under alkaline conditions. Journal of Basic Microbiology; Date of Electronic Publication: 2013 May 17
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