Can cells be grown in exogenous supply of Malonyl-CoA and Malate? Need Help

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Can cells be grown in exogenous supply of Malonyl-CoA and Malate? Need Help

ExpertScie Offline

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Posts: 133
Threads: 29
Likes Received: 3 in 3 posts
Likes Given: 0
Joined: Sep 2012
BioEXP: 0Bx

10-17-2012, 03:01 AM (This post was last modified: 10-17-2012, 03:03 AM by ExpertScie.)

(10-17-2012, 01:34 AM)billamin Wrote: sir, to be specific, i will be experimenting with trypanosomatids.so the problem of gram +ve/-ve will not arise.i want to inhibit the first step of FA synthesis where acetyl-CoA is converted to malonyl-Coa. So to check the effect, i want to supply malonyl-CoA and see if the phenotype is recovered to that of untreated cells.so, will this approach work?

Dear Billamin,
If possible for your project then try supplementing the culture media
with Oleate. It has the formula CH3(CH2)7CH=CH(CH2)7COOH.
It will work, good luck !