10-17-2012, 12:24 AM
(This post was last modified: 10-17-2012, 12:33 AM by Administrator.)
Dear Billamin,
Welcome to the site ! You can post all your queries related to biotechnology & other biological science in this forum.
As you may be know that Malonyl-CoA is the precursor for the de novo synthesis and elongation of fatty acids chains. Also these are used in formation of flavonoids,stilbenoids, isoflavonoids etc and in many other malonylated compounds, including d-amino acids, malonylated 1-aminocyclopropane-1-carboxylic acid, and flavonoid glycosides.
The answer of your query "Can cells be grown in exogenous supply of Malonyl-CoA and Malate", is No if they are gram negative organisms (cells)or any cells with more lipid content in their membranes.
Since Malate are anions (here consider it in terms of polarity - as we are thinking of exogenous supply-ONLY) and are unable to pass through the lipid bilayer of membranes and hence require carriers (eg 2-oxoglutarate/malate carrier protein)for the same.Therefore if you are thinking of cell with more lipid membrane example (Gram Negative microorganisms ) this may not be possible without carriers and if the only source of growth is this.
Now if you are thinking of exogenous supply (ONLY) of Malonyl-CoA , then it may further depends on levels of other influencing factors like the activity of AMPK within that cells. AMPK as is activated by increase in AMP/ATP ratio, which is indication of fuel requirement or fasting or exercise also influence balancing or absorption of Malonyl Co-A.
Also Malonyl CoA as you may know is indication of plenty (sufficient energy). Therefore,its balance or exogenous source requirement/absortion within cell depends on another factor at the same time.
To check the effect of inhibition of pathways in animals/humans,you may go for profile of Urine organic acids and compare it with standard /normal.
If small animals then you may study effect by evaluating it with decrease in body temperature or weight loss etc.
Further, specify your queries & Wish you best of luck for your project !
Welcome to the site ! You can post all your queries related to biotechnology & other biological science in this forum.
As you may be know that Malonyl-CoA is the precursor for the de novo synthesis and elongation of fatty acids chains. Also these are used in formation of flavonoids,stilbenoids, isoflavonoids etc and in many other malonylated compounds, including d-amino acids, malonylated 1-aminocyclopropane-1-carboxylic acid, and flavonoid glycosides.
The answer of your query "Can cells be grown in exogenous supply of Malonyl-CoA and Malate", is No if they are gram negative organisms (cells)or any cells with more lipid content in their membranes.
Since Malate are anions (here consider it in terms of polarity - as we are thinking of exogenous supply-ONLY) and are unable to pass through the lipid bilayer of membranes and hence require carriers (eg 2-oxoglutarate/malate carrier protein)for the same.Therefore if you are thinking of cell with more lipid membrane example (Gram Negative microorganisms ) this may not be possible without carriers and if the only source of growth is this.
Now if you are thinking of exogenous supply (ONLY) of Malonyl-CoA , then it may further depends on levels of other influencing factors like the activity of AMPK within that cells. AMPK as is activated by increase in AMP/ATP ratio, which is indication of fuel requirement or fasting or exercise also influence balancing or absorption of Malonyl Co-A.
Also Malonyl CoA as you may know is indication of plenty (sufficient energy). Therefore,its balance or exogenous source requirement/absortion within cell depends on another factor at the same time.
To check the effect of inhibition of pathways in animals/humans,you may go for profile of Urine organic acids and compare it with standard /normal.
If small animals then you may study effect by evaluating it with decrease in body temperature or weight loss etc.
Further, specify your queries & Wish you best of luck for your project !
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