This is post no. 1 under the main topic.
| 11-10-2016, 04:31 AM
(This post was last modified: 11-10-2016, 04:33 AM by priyansha sinha.)
I have a question regarding vector DNA change . when some foreign gene (like gene for pest resistance) is inserted into a vector say agrobacterium tumefaciens so as to transfer this gene into plants , doesn't it harm the mechanism of agrobacterium? Doesn't it make agrobacterium resistant ?
kindly assist me giving answer of this question.
This is post no. 3 under the main topic.
| 12-28-2016, 08:49 PM
when we want to insert a new gene in host....ist of all the vector. having gene of intrest is inactive. and tha molecules. which. are able to gene regulations. and exprassion for gene of intrest are not present. in agrobaterium....these are olny preset in plants....so that the gene of interest. never shows it funcation in bacteria....
This is post no. 5 under the main topic.
| 12-29-2016, 04:30 AM
m just giving. u general......nore study u should. check book
This is post no. 6 under the main topic.
| 01-09-2017, 06:57 PM
I know Agrobacterium tumefaciens is a soil borne bacteria that causes gall crown(tumor) in plant. It uses a specific gene to alter the plant cell genome resulting in an uncontrolled cell divisions (tumor). So if this gene is removed and replaced with a resistance gene, this will take control of the plant genome resulting in the plant cell developing or becoming pest resistance. But note that the bacteria must be introduced in a part of plants undergoing cell division such as the meristem