02-11-2017, 06:24 PM
(02-11-2017, 05:29 PM)Shubhangi Srivastava Wrote: If the pH of a protein is reduced to 1.5....below it's isoelectric point,which of it's group would be ionisable ?At isoelectric point, amino acid exists in Zwitterion state...Reducing the pH below isoelectric point means addition of H+ ions to the medium....this would allow -COO(-) ion of zwitterion form to pick up the H+ ion and become -COOH, leaving behind -NH3(+) ionic form....
i)COOH
ii)NH2
iii)Both of these
iv)Both would be neutral
-NH2 is in ionized state as a result.