[dipak0mukherjee]

sir,
iisc has published the syllabus for gate 2016 biotechnology..analytical techniques ..this part has been added..which book should i follow for this part? in biochemistry neucleotide metabolism ,amino acid metabolism ,lipid metabolism are also added...:-( and in the part of bioprocess engineering unit operations in liquid liquid and solid liquid has been added..which book should i follow for this ?
BT
Biotechnology (NEW SYLLABUS)
Section 1: Engineering Mathematics
Linear Algebra: Matrices and determinants, Systems of linear equations, Eigen values and
Eigen vectors. Calculus: Limit, continuity and differentiability, Partial derivatives, Maxima
and minima, Sequences and series, Test for convergence, Fourier Series. D
ifferential
Equations: Linear and nonlinear first order ODEs, higher order ODEs with constant
coefficients, Cauchy’s and Euler’s equations, Laplace transforms, PDE
-
Laplace, heat and
wave equations. Probability and Statistics: Mean, median, mode and standar
d deviation,
Random variables, Poisson, normal and binomial distributions, Correlation and regression
analysis. Numerical Methods: Solution of linear and nonlinear algebraic equations,
Integration of trapezoidal and Simpson’s rule, Single and multistep met
hods for differential
equations.
Section 2: General Biotechnology
Biochemistry:
Biomolecules
-
structure and functions; Biological membranes, structure,
action potential and transport processes; Enzymes
-
classification, kinetics and mechanism
of action; Basi
c concepts and designs of metabolism (carbohydrates, lipids, amino acids
and nucleic acids) photosynthesis, respiration and electron transport chain; Bioenergetics
Microbiology
:
Viruses
-
structure and classification
;
Microbial classification and
diversity(bacterial, algal and fungal)
;
Methods in microbiology
;
Microbial growth and
nutrition
;
Aerobic and anaerobic respiration
;
Nitrogen fixation
;
Microbial diseases and
host
-
pathogen interaction
Cell Biology
: Prokaryotic and eukaryotic cell structure
;
Cell cycle and cell growth control
;
Cell
-
Cell communication, Cell signaling and signal transduction
Molecular Biology and Genetics
: Molecular structure of genes and chromosomes;
Mutations and mutagenesis; Nucleic acid replication, transcription, transla
tion and their
regulatory mechanisms in prokaryotes and eukaryotes; Mendelian inheritance; Gene
interaction; Complementation; Linkage, recombination and chromosome mapping; Extra
chromosomal inheritance; Microbial genetics (plasmids, transformation, transd
uction,
conjugation); Horizontal gene transfer and Transposable elements; RNA interference; DNA
damage and repair; Chromosomal variation; Molecular basis of genetic diseases
Analytical Techniques:
Principles of microscopy
-
light, electron, fluorescent and
confocal
;
Centrifugation
-
high speed and ultra; Principles of spectroscopy
-
UV, visible, CD, IR, FTIR,
Raman, MS,NMR
;
Principles of chromatography
-
ion exchange, gel filtration, hydrophobic
interaction, affinity
,
GC,HPLC, FPLC; Electrophoresis
;
Microarray
Immunology
: History of Immunology;
Innate, humoral and cell mediated immunity;
Antigen
;
Antibody structure and function
;
Molecular basis of antibody diversity; Synthesis
of antibody and secretion; Antigen
-
antibody reaction; Complement
;
Primary and
secon
dary lymphoid organ; B and T cells and macrophages
;
Major histocompatibility
complex (MHC)
;
Antigen processing and presentation
;
Polyclonal and monoclonal
antibody
;
Regulation of immune response; Immune tolerance
;
Hypersensitivity;
Autoimmunity; Graft vers
us host reaction.
Bioinformatics:
Major bioinformatic resources and search tools; Sequence and structure
databases
;
Sequence analysis (biomolecular sequence file formats, scoring matrices,
sequence alignment, phylogeny);Data
mining and analytical tools for genomic and
proteomic studies; Molecular dynamics and simulations (basic concepts including force
fields, protein
-
protein, protein
-
nucleic acid, protein
-
ligand interaction)
Section 3: Recombinant DNA Technology
Restriction
and modification enzymes; Vectors; plasmid, bacteriophage and other viral
vectors, cosmids, Ti plasmid, yeast artificial chromosome; mammalian and plant
expression vectors; cDNA and genomic DNA library; Gene isolation, cloning and
expression ; Transposons
and gene targeting; DNA labeling; DNA sequencing; Polymerase
chain reactions; DNA fingerprinting; Southern and northern blotting; In
-
situ hybridization;
RAPD, RFLP; Site
-
directed mutagenesis; Gene transfer technologies; Gene therapy
Section 4: Plant and An
imal Biotechnology
Totipotency; Regeneration of plants; Plant growth regulators and elicitors; Tissue culture
and Cell suspension culture system: methodology, kinetics of growth and, nutrient
optimization; Production of secondary metabolites by plant susp
ension cultures; Hairy root
culture; transgenic plants; Plant products of industrial importance
Animal cell culture; media composition and growth conditions; Animal cell and tissue
preservation; Anchorage and non
-
anchorage dependent cell culture; Kinetics
of cell
growth; Micro & macro
-
carrier culture; Hybridoma technology; Stem cell technology;
Animal cloning; Transgenic animals
Section 5: Bioprocess Engineering and Process Biotechnology
Chemical engineering principles applied to biological system, Princip
le of reactor design,
ideal and non
-
ideal multiphase bioreactors, mass and heat transfer; Rheology of
fermentation fluids, Aeration and agitation; Media formulation and optimization; Kinetics
of microbial growth, substrate utilization and product formation
; Sterilization of air and
media; Batch, fed
-
batch and continuous processes; Various types of microbial and
enzyme reactors; Instrumentation control and optimization; Unit operations in solid
-
liquid
separation and liquid
-
liquid extraction; Process scale
-
up
, economics and feasibility
analysis
Engineering principle of bioprocessing
-
Upstream production and downstream;
Bioprocess design and development from lab to industrial scale; Microbial, animal and
plant cell culture platforms; Production of biomass and
primary/secondary metabolites;
Biofuels, Bioplastics, industrial enzymes, antibiotics; Large scale production and
purification of recombinant proteins; Industrial application of chromatographic and
membrane based bioseparation methods; Immobilization of bi
ocatalysts (enzymes and
cells) for bioconversion processes; Bioremediation
-
Aerobic and anaerobic processes for
stabilization of solid / liquid wastes