11-27-2012, 02:02 PM
hi sir,
I search answer in web and finally i got it answer. But I did not understand I have poor english knowledge so any grammatical mistakes sorry. Can you explain the answer
Answer is 4096 bp
Expected frequency of cleavage depends on the complexity of the recognition sequence.
For instance, for the common 6-base cutter, EcoRI, the frequency of its recognition sequence (GAATTC) is ¼ x ¼ x ¼ x ¼ x ¼ x ¼ = once per 4,096 base pairs.
For the more relaxed 6 base cutter, BsrFI (PuCCGGPy), this is ½ x ¼ x ¼ x ¼ x ¼ x ½ = once every 1024 base pairs.
I search answer in web and finally i got it answer. But I did not understand I have poor english knowledge so any grammatical mistakes sorry. Can you explain the answer
Answer is 4096 bp
Expected frequency of cleavage depends on the complexity of the recognition sequence.
For instance, for the common 6-base cutter, EcoRI, the frequency of its recognition sequence (GAATTC) is ¼ x ¼ x ¼ x ¼ x ¼ x ¼ = once per 4,096 base pairs.
For the more relaxed 6 base cutter, BsrFI (PuCCGGPy), this is ½ x ¼ x ¼ x ¼ x ¼ x ½ = once every 1024 base pairs.
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