11-23-2012, 04:59 PM
(This post was last modified: 11-23-2012, 05:01 PM by ExpertScie.)
Dear Kanagasundar87,
Can you please specify the chromosome in question like its form, source etc.? Is it of human or any other animal etc?
This will help to calculate the same. Or you may share the course type on which these objective questions are being asked. This will give hint to identify the answer.
Background::
As you may know, chromosome or even plasmids form / shape contribute to number of fragments. Example whenever these are circular pieces of DNA, the fragment number of equals the number of cuts from the restriction enzymes. If plasmid or chromosome is cut at one place by one restriction enzyme then the fragment will be only one. Cutting the circle one will yields only one fragment. If the restriction cuts in two places, it will result in two fragments; with three places, three fragments and so on etc.
In case of linear chromosomes, the situation is slightly different. Cutting a linear chromosome at one place yield two fragments. Similarly cutting it at two places results in three fragments etc. So the number of fragments depends upon the type of cut as well as the original base pair of the chromosome in question (That is chromosome source and form).
A restriction map of a chromosome will indicate all of the cuts as made by the restriction enzymes. One can count the spaces or gap between cuts to find out the number of fragments that are produced.
Also partial DNAse/RE digestion of chromatin reveals its nucleosome structure. Because DNA portions of nucleosome core particles are more accessible for RE(Restriction Enzyme type I-IV) than Dnase. DNA gets digested into fragments equal to multiplicity of distance between nucleosomes (like 180, 360, 540 base pairs etc.).
Hope this will help you to find out the answer. Or please specify the RE type, its source, Chromosome in question to identify the exact answer.
Can you please specify the chromosome in question like its form, source etc.? Is it of human or any other animal etc?
This will help to calculate the same. Or you may share the course type on which these objective questions are being asked. This will give hint to identify the answer.
Background::
As you may know, chromosome or even plasmids form / shape contribute to number of fragments. Example whenever these are circular pieces of DNA, the fragment number of equals the number of cuts from the restriction enzymes. If plasmid or chromosome is cut at one place by one restriction enzyme then the fragment will be only one. Cutting the circle one will yields only one fragment. If the restriction cuts in two places, it will result in two fragments; with three places, three fragments and so on etc.
In case of linear chromosomes, the situation is slightly different. Cutting a linear chromosome at one place yield two fragments. Similarly cutting it at two places results in three fragments etc. So the number of fragments depends upon the type of cut as well as the original base pair of the chromosome in question (That is chromosome source and form).
A restriction map of a chromosome will indicate all of the cuts as made by the restriction enzymes. One can count the spaces or gap between cuts to find out the number of fragments that are produced.
Also partial DNAse/RE digestion of chromatin reveals its nucleosome structure. Because DNA portions of nucleosome core particles are more accessible for RE(Restriction Enzyme type I-IV) than Dnase. DNA gets digested into fragments equal to multiplicity of distance between nucleosomes (like 180, 360, 540 base pairs etc.).
Hope this will help you to find out the answer. Or please specify the RE type, its source, Chromosome in question to identify the exact answer.
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