11-13-2013, 07:53 AM
What are the steps in identification homologous genes between Arabidopsis and tomato. I need to identify the homologous genes in tomato, do multiple alignment, build phylogenetic tree, and find analyze synteny.
2) What kind of alignment should I used, NJ or ML?
3) How to identify synthenic block for my gene of interest
4) is it acceptable if I BlastP the amino acid sequence from Arabidopsis as a query and the first hit has E-value of 9e-19 and 42% of identity?
2) What kind of alignment should I used, NJ or ML?
3) How to identify synthenic block for my gene of interest
4) is it acceptable if I BlastP the amino acid sequence from Arabidopsis as a query and the first hit has E-value of 9e-19 and 42% of identity?