[warmbaby]

Please I am about to carry out a few enzyme assays. 

First I would like to know which are the most common enzymes carried out in organic media. 

Secondly, what is the best way to carry out a protein purification to remove the solvents and what is the acceptable maximum time to keep the protein at 4 degree before performing enzyme test? Or I wonder what is the common option in your lab, test before it loses activity at 4 degree or store the protein at -80 degree for long term use? 

Thanks