Biotechnology Forums - Biotechnology Products

[Feedback Request] Drug Bible - The complete drug guide (iOS/Android)

Sat, 08 May 2021 04:43:07 +0000

Hi All,

I'm the developer of the "Drug Bible" and other apps such as Smart Pill ID ( an AI powered pill identifier).

Available on the App Store and Play Store.

I worked with users in the FDA, NIH, and other health organizations to complete the ultimate drug guide. This handy tool has all the information you will ever need as a consumer, prescriber, or researcher. The Drug Bible has information on over 100,000 prescription and over the counter drugs, both brand name and generics.

Quickly search for drugs or by application to view drug images, labels, documents (research, reviews, letters, and others), as well as clinical and consumer information. Consumer information includes general information, side effects, news, and drug prices. Clinical information includes the following information:

Adverse Reactions
Clinical Pharmacology
Contraindications
Description
Dosage and Administration
Drug Interactions
How Supplied
Indications and Usage
Mechanism of Action
Overdosage
Pediatric Use
Pregnancy

Once a medical application has been chosen, you may filter the drugs by Brand Name, Generic Name, Substance Name, Manufacturer Name, Chemical Structure Class, Established Pharmacologic Class, Pharmacological Class, and Mechanism of Action. Drug information is updated monthly.

Any and all feedback is much appreciated!

How to Improve the Fusion Rate of Monoclonal Antibodies

Tue, 27 Apr 2021 08:36:04 +0000

Abstract: At the beginning of performing monoclonal antibody test, the failure of cell fusion or low fusion rate happens sometimes. Now, some factors for influencing fusion rate are summarized, which may be helpful for experimenters.

Keywords: Monoclonal Antibodies, Fusion Rate, Antibodies Fusion

1. Blood Titer Test
Before the fusion, the mouse blood should be collected for testing the titer. The titer higher than 20,000 is suitable for the fusion. The fusion effect may not be satisfactory if the titer is lower than 20,000.

2. Cell State
The state of myeloma cells SP2/0 is very important and should be kept without any pollution and large scale death. The logarithmic growth phase of cells should be maintained before the fusion.

3. PEG and Fatal Bovine Serum
The fusion rate depends on the quality of PEG and fatal bovine serum which should be subject to the pilot experiment screening between different brands and batch numbers. Selecting the best quality PEG and fatal bovine serum can ensure the successful rate of fusion efficiently.

4. Cell Ratio
During the fusion, the ratio of SP2/0 and splenocytes should be noticed as well. Although 10:1-1:10 is applicable for the successful fusion, the best fusion efficiency is between 2:1-1:2. No matter which ratio, the two kinds of cells don't contain serum components and should be mixed well. During the fusion process, the fusion tube should be always rotated. Dropping PEG and terminating the fusion should be slowly first and fast afterwards to avoid the great impact on the cell.

5. Feeder Layer Cells
Feeder layer cells can also influence the fusion effect. Feeder cells of hybridoma can be chosen from mouse peritoneal macrophages, splenocytes and thymocytes. The peritoneal macrophages is the best choice. Meanwhile, the amount of feeder cells in each well should be also noticed. Excessive cells will fight for medium nutrition, making the growth of hybridoma cells difficult to maintain. Deficient cells can't serve as the feeder layer. Hence, only the reasonable cell amount can achieve the good effect.

6. Medium Additives
The intelligent use of some additives has a good prospect. E.g. the hybridoma cell culture medium additive developed by FineTest can efficiently extend the service life of medium, improve the growth speed of hybridoma cells and increase fusion rate. Under unsatisfactory experimental conditions, the fusion rate can be still maintained well.

Figure 1. Hybridoma Cell Culture Medium Additive(100X)

Figure 2. Comparison between fusion cells without medium additives and hybridoma cells with medium additives.

7. Conclusion
There are other factors for influencing fusion rate. E.g.: the number of cells in each well after the fusion, centrifugal rotational speed and time, the strength for pipetting cells etc. During the actual experimental process, it's important to accumulate more experience.

I need help

Tue, 07 Aug 2018 08:26:22 +0000

want to know about IHC Protocol? Guide me.

Arbuscular Mycorrhizal Fungi

Sat, 19 May 2018 03:37:37 +0000

Good morning, I'm Sieu. I need to help from scientific communities about what's method for examine the tolerance of arbuscular mycorrhizal fungi colonized in saline-alkaline soils in paddy fields? Thank you so much?

Biotechnology

Sun, 25 Feb 2018 15:51:37 +0000

I'm pursuing my btech biotechnology engineering and I'm totally confused of what to do next and in this field !

Cloning of animal

Sat, 23 Dec 2017 10:36:04 +0000

Is it a good idea to clone animals for pharmaceutical use, if yes I need a impressive protocols to carry out my research work

Medical coding

Mon, 14 Aug 2017 07:16:22 +0000

After doing b.tech in biotechnology, doing medical coding is good ? Guide me

My Career in Biotechnology

Mon, 10 Jul 2017 14:42:10 +0000

I have completed my B.Sc in Biotechnology an year back. I would like to know about my various career opportunities and job Oriented courses that I should opt in Chennai.

Mosquito killer natural ingredient and organic pesticide formula

Tue, 27 Jun 2017 13:53:47 +0000

How to make mosquito killer form natural /herbal ingredients (essential oil,powder,flower ,etc ) and research technology for commercializon

Mosquito killer natural ingredient

Thu, 15 Jun 2017 20:01:11 +0000

I need mosquito repellent and killer formula.mosquito killer agorbatti making,coil,spray,mat etc making ingredient and formulation

Selling of microbes

Fri, 21 Apr 2017 01:06:33 +0000

Guys I have an doubt it is possible or legal to sell micro organism in online and send it through parcel

I need a help

Tue, 18 Apr 2017 12:53:45 +0000

Sir/mam
I am doing my Mtech first year biotechnology programme.I am interested in doing project by using plant source as therapy for treating the provoking disease by protein extraction and purification.But i dont have idea in screening out plant sources and to choose the disease.. Can anyone help me??give suggestions so that i can get through and came out with better idea!! Please

I need a help !

Tue, 07 Mar 2017 10:38:04 +0000

I'm studying pharmacy , and I've to make a project for biotechnology subject ...I need some help in that !

Biofertilizer

Mon, 06 Mar 2017 15:34:13 +0000

I have planned to start a biofertilizer production unit ...what is the best one in biofertilizer

Biotechnology entrepreneurship

Thu, 26 Jan 2017 14:41:01 +0000

Iam pursuing my bachelor degree in biotechnology and I wish to be a entrepreneur in this field,but I have no idea how and where to go forward