Biotechnology Forums - All Forums

How accurate tridot test kit are?

Fri, 29 Oct 2021 10:50:01 +0000

How reliable is the TRI-DOT HIV test? Is there a case where a person is infected but the TRI-DOT showed a negative result? Recently I have gotten to know through my diagnostic lab colleague about this. he is currently using HIV Test Kit provided by a company J Mitra. According to him, the results are good & accurate. just want to suggest.

CRISPR/Cas

Fri, 24 Sep 2021 10:55:05 +0000

May someone please help me with this:

You observed that the CRISPR knock-out of protein X either promotes or
represses RNA polymerase II transcription, depending on the gene. Which
experiments would you carry out to reveal the mechanism for this
differential activity?

Thank you

CRISPR/Cas

Fri, 24 Sep 2021 10:52:12 +0000

Challenges in translating discoveries or inventions from the lab into the market

Tue, 03 Aug 2021 09:12:54 +0000

What problems did you face when you tried to commercialize your scientific discoveries or inventions from the laboratory?

dCas9(D10A) and AncBE4max for Polynesian Albinism single nucleotide conversion

Tue, 29 Jun 2021 21:30:56 +0000

Hello!

I'm working on a personal project to convert a Guanine into an Adenine in this specific albinism causing allele: https://www.ncbi.nlm.nih.gov/gene?Db=gen...;Term=4948

The mutation is at 2324 in the code.
My plan is to use a double nickase dCas9(D10A) to bring the AncBE4max to that site and convert the Guanine into Adenine while in-vivo.

I took inspiration from this article in particular: https://www.nature.com/articles/jhg2009130?proof=t

And this one: https://www.mdpi.com/2073-4409/9/7/1690/htm

As well as this article: https://www.nature.com/articles/s41467-019-11514-0

I haven't yet built a plasmid from scratch and am trying to use GenSmart Design to do so, but am not yet able to figure out how to delete things from the prebuilt plasmid sections. I am hoping to get some aide into what programs I should be using to build my plasmid as well as any know-how into how this works and if it can work.

Using the sgrna design tools online with the basic PAM of NGG, I haven't been able to find sites close enough to the edit zone to seem viable. I am trying to keep it below a span of 40 nucleotides, but I also don't have a good idea of what I'm doing. Any help with explaining how I should be going about this would be wonderful.

Due to certain personal circumstances, I am unable to continue my education at university and am trying to find other ways to continue my education into this field. Any tips for that would also be greatly appreciated.

Godspeed,

- Drig

Need Lab (academic or commercial) Routinely Running Akt activation ELISA Assays

Thu, 24 Jun 2021 14:53:30 +0000

The title says it all. I need to find a lab already setup to do routine ELISA assays for Akt phosphorylation in PBMCs. Something along the lines of the ThermoFisher Akt(pS473) assay kits or equivalent. All the labs I have canvassed are unfamiliar and require method transfer and validation which turns out to be triple the cost of running the assays themselves. This would be non-GLP, could be academic or commercial lab and would initially be about 300 samples in duplicate.

Thanks in advance

John