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Understanding Flow Cytometry in a Simple Way!
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During the past ten decades the utilization of flow cytometry in the clinical labs has increased substantially. This is due to the development of less expensive, user friendly smaller instruments and also advancement in various clinical applications.

Immunophenotyping – It is the most frequently utilized application that is performed on the flow cytometer. This technique is used to identify and quantify heterogeneous populations of cells normally blood, lymph or bone marrow. In clinical labs it is useful in the diagnosis of leukemia and lymphomas (haematological malignancies).

Cell Sorting - The cell sorter is a flow cytometer that is specialized with the ability to isolate cells of interest by physical means into different collection tubes. This cytometer characterizes and interrogates every cell as it passes through a laser. The sorter then utilizes sophisticated and advanced electronics and fluidics to recognise and kick the cells of interest from the fluid stream into a test tube.

Cell Cycle Analysis – The four phases of the cell cycle can be analysed by Flow cytometry utilizing the fluorescent dyes. In addition to the determination of phases of cell cycle, this flow cytometry assay can also measure aneuploidy in the cells linked with chromosomal abnormalities.

Apoptosis – Also called as programmed cell death, is a usual part of the eukaryotic cell cycle. The two distinct forms of cell death that is apoptosis and necrosis can be differentiated and distinguished by flow cytometry by means of differences in biochemical, morphological and molecular events that occur in the dying cells.

Cell Proliferation Assays - Cell proliferation assays are extensively utilized in cell biology to determine metabolic activity of the cell in response to stimuli such as cytokines, growth factors and other components of the media. The flow cytometry is capable of measuring the cell proliferation by labeling the resting cells with a fluorescent dye of cell membrane, CFSE (carboxyfluorescein succinimidyl ester).

Intracellular Calcium Flux – By means of signal transduction pathways, the cells interact with their environment and also with each other. The flow cytometer can monitor the of calcium flux into the cell and quantify the extent to which cells are responding to the stimuli.

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RE: Understanding Flow Cytometry in a Simple Way! - by adimed - 10-05-2013, 02:05 PM
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