[S Ghosh]

Can you please explain the following in a simple language- When we isolate nucleic acid from any tissue like cheek cells and go for restriction endonuclease treatment, we get huge numbers of fragments. These fragments represent portions of DNA from different chromosomes. Now if we do electrophoresis of the same, we will gey bands at different distances, based on sizes. Now I have three questions- 01. How we identify and isolate the gene of interest from here. 02.How we allocate the fragments containing genes to respective chromosomes. 03. For long genes, how do we ascertain that the entire gene is represented by restriction endonuclease treatment. Thanks and regards.