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Thread: Radioimmunotherapy provides hope of HIV cure
Post: RE: Radioimmunotherapy provides hope of HIV cure
Post: RE: Radioimmunotherapy provides hope of HIV cure
A restore to health for human immunodeficiency virus (HIV) could in a little while become reality, according to a new study. Using a groundbreaking therapy method involving so-called radioimmunotherap...
Author: brijnbhatt
Forum:
News
01-08-2014, 05:42 AM
Thread: Detection of Transgenic Animals
Post: RE: Detection of Transgenic Animals
Post: RE: Detection of Transgenic Animals
Other than the method has been described:
I found, there are methods which has been used for making transgenic animal and there are special techniques to identify them. Making of genetically engineer...
Author: brijnbhatt
Forum:
Cloning
01-08-2014, 05:10 AM
Thread: Want to Buy Primary Anitbody?
Post: RE: Want to Buy Primary Anitbody?
Post: RE: Want to Buy Primary Anitbody?
Hi,
I think the antibody from different companies are different. Means some antibodies are working good from one company but from same company other antibodies are not good. I have found most of the ...
Author: brijnbhatt
Forum:
Biotechnology Products
12-31-2013, 11:29 PM
Thread: Stem Cell: An Answer to Our Prayers?
Post: RE: Stem Cell: An Answer to Our Prayers?
Post: RE: Stem Cell: An Answer to Our Prayers?
Stem cells are the body's raw materials. Cells from which all other cells with specialized functions are generated. Under the right conditions in the body or a laboratory, stem cells divide to form d...
Author: brijnbhatt
Forum:
Stem Cells
12-31-2013, 11:11 PM
Thread: Instability of Stem Cells
Post: RE: Instability of Stem Cells
Post: RE: Instability of Stem Cells
The steady experience of cells to endogenous and exogenous agents that inflict DNA damage requires active repair processes to get rid of potentially mutagenic events in stem cells leading to cancer. T...
Author: brijnbhatt
Forum:
Stem Cells
12-31-2013, 10:45 PM
Thread: Cancer Immunotherapy Set for Human Trials
Post: RE: Cancer Immunotherapy Set for Human Trials
Post: RE: Cancer Immunotherapy Set for Human Trials
Immunotherapy is a most advanced cancer therapy
Radiation, chemotherapy, and surgery are the conventional methods in the fight against cancer. Radiation was discovered in the 1800's by Marie Curie. C...
Author: brijnbhatt
Forum:
Gene and Cell therapy
12-31-2013, 10:21 PM
Thread: Acid for TMB in Elisa Kits
Post: RE: Acid for TMB in Elisa Kits
Post: RE: Acid for TMB in Elisa Kits
Hello
Stopping reagent for peroxidase and TMB: 3 M HCl or 3 M H2SO4
you need to use 50ul for total 450ul reaction. so final concentration will be ~0.33 M. Final concentration can vary from 0.2M to ...
Author: brijnbhatt
Forum:
Questions and Answers
12-31-2013, 09:15 PM
Thread: gfp protein purification
Post: RE: gfp protein purification
Post: RE: gfp protein purification
Dear Jac,
I can give you concept for purifying GFP protein,
Check the pI of GFP it is around 5.0 so you use a buffer (Tris-Cl 50mM, pH 7.5) and purify it from DEAE column (Anionic Exchange) as at ...
Author: brijnbhatt
Forum:
Questions and Answers
12-31-2013, 08:59 PM
Thread: A Journey Through A Cell
Post: RE: A Journey Through A Cell
Post: RE: A Journey Through A Cell
Bacterial Cells and Its Importance:
They are distinct to human beings as living belongings can be, but bacteria are essential to human life and life on Earth. Even though they are infamous for their ...
Author: brijnbhatt
Forum:
Genetic Engineering
12-31-2013, 08:28 PM
Thread: Targeted Gene Delivery: Future of High Efficiency Gene Therapy
Post: RE: Targeted Gene Delivery: Future of High Efficie...
Post: RE: Targeted Gene Delivery: Future of High Efficie...
Targeted Gene Therapy in Cancer:
In recent past the thought of using gene therapy as a modality in the handling of diseases other than genetically inherited, a monogenic disorder has taken origin. T...
Author: brijnbhatt
Forum:
Gene and Cell therapy
12-31-2013, 08:02 PM
Thread: Proteomics - New biology in the field of Science
Post: RE: Proteomics - New biology in the field of Scien...
Post: RE: Proteomics - New biology in the field of Scien...
Clinical Proteomics:
The detailed information of the composition and activities of urine proteins could offer novel insights into normal renal physiology. Although proteomic studies have identified a...
Author: brijnbhatt
12-31-2013, 07:43 PM
Thread: Human Disease Causing Bacteria
Post: RE: Human Disease Causing Bacteria
Post: RE: Human Disease Causing Bacteria
Human Bacterial Disease by Gram Positive Cocci:
Toxic Shock Syndrome (TSS):
The pathogenic species is Staphylococcus aureus (aure = gold, golden), which causes most hospital-acquired infections. St...
Author: brijnbhatt
12-31-2013, 07:15 PM
Thread: Role of Adjuvants in Immunology
Post: RE: Role of Adjuvants in Immunology
Post: RE: Role of Adjuvants in Immunology
The Immunization with Adjuvants can be used for a variety of purposes and some of them are listed here:
• It is to improve the immunogenicity of highly purified or recombinant antigens (protein or pep...
Author: brijnbhatt
12-31-2013, 06:43 PM
Thread: The Terrifying Rise of Antibiotic Resistance
Post: RE: The Terrifying Rise of Antibiotic Resistance
Post: RE: The Terrifying Rise of Antibiotic Resistance
Multi Drug resistance in Mycobactrium tuberculosis
The bacteria which cause disease called tuberculosis (TB) is gram positive bacteria and it can develop resistance to the antimicrobial drugs used t...
Author: brijnbhatt
12-31-2013, 03:48 AM
Thread: Ways to produce Monoclonal Antibodies
Post: RE: Ways to produce Monoclonal Antibodies
Post: RE: Ways to produce Monoclonal Antibodies
Specialized methods for monoclonal antibody production: (Decoy Method)
To study the embryonic stem cell and their differentiation pattern, it is very impotent to understand the proteins which are inv...
Author: brijnbhatt
Forum:
Cloning
12-31-2013, 03:02 AM
Thread: Cloning multiple genes in plant vector
Post: RE: Cloning multiple genes in plant vector
Post: RE: Cloning multiple genes in plant vector
Dear member,
I saw your question just now. Well, you can clone multiple genes in same vector. As it is Eukaryota system, it is important to have following things for expression.
1- Suitable promo...
Author: brijnbhatt
Forum:
Cloning
12-31-2013, 02:16 AM
Thread: Isolation and purification of plasmids
Post: RE: Isolation and purification of plasmids
Post: RE: Isolation and purification of plasmids
There are quick and easy method to isolate Plasmid from Bacteria here I have elaborated the protocol for isolation of Plasmid from different type of bacteria.
1. Boiling method for small plasmids in...
Author: brijnbhatt
Forum:
Genetic Engineering
12-30-2013, 02:13 AM
Thread: Cure and Causes of Alzheimer's Disease: A Must Read!
Post: Familial Alzheimer’s Disease
Post: Familial Alzheimer’s Disease
Alzheimer's disease is a disorder caused by neurological problems in which the death of brain cells causes memory loss and cognitive decline. A neurodegenerative type of dementia, the disease starts m...
Author: brijnbhatt
Forum:
Diseases and Diagnostics
12-29-2013, 05:59 AM
Thread: Designer babies
Post: Designer babies
Post: Designer babies
It is new era where babies can be designed with the help of In vitro fertilization. It has helped for many difficulties with having children. However, it raises a number of ethical issues, one of whic...
Author: brijnbhatt
Forum:
Genetic Engineering
12-29-2013, 05:28 AM
Thread: Genetic disorder and its diagnosis
Post: Genetic disorder and its diagnosis
Post: Genetic disorder and its diagnosis
Disease caused genetic disorder are abnormality in an individual's DNA. These abnormalities can range from a small mutation in a single gene to the addition or deletion of an entire chromosome or set ...
Author: brijnbhatt
Forum:
Gene and Cell therapy
12-29-2013, 04:54 AM
Thread: Gene cloning for expression
Post: Gene cloning for expression
Post: Gene cloning for expression
Cloning of a gene for expression purpose (in bacterial, yeast, mammalian or other systems) involves many steps. Depending up on the information available on the gene of interest, various methods can b...
Author: brijnbhatt
Forum:
Genetic Engineering
12-27-2013, 09:17 PM
Thread: Stem Cell and Gene therapy
Post: Stem Cell and Gene therapy
Post: Stem Cell and Gene therapy
Gene Therapy is advisable for genetic diseases which cannot be cured. Here stem cells are very important and most potent cells which can be used for gene therapy and after that they can be transplante...
Author: brijnbhatt
Forum:
Gene and Cell therapy
12-27-2013, 05:34 PM
Thread: Parameters for expression of protein
Post: RE: Parameters for expression of protein
Post: RE: Parameters for expression of protein
Dear Ikariotis,
I saw your post just now, I am a molecular biologist and I have tried expression of many mammalian proteins in bacterial system. I found expression will depend upon many factors and ...
Author: brijnbhatt
Forum:
Genetic Engineering
12-24-2013, 02:54 AM