CRISPR-Cas9 gene deletion in Glycine max - Printable Version +- Biotechnology Forums (https://www.biotechnologyforums.com) +-- Forum: Information (https://www.biotechnologyforums.com/forum-16.html) +--- Forum: Questions and Answers (https://www.biotechnologyforums.com/forum-37.html) +--- Thread: CRISPR-Cas9 gene deletion in Glycine max (/thread-8409.html) |
CRISPR-Cas9 gene deletion in Glycine max - gia.pa - 06-21-2018 Hi, i'm a bachelor student seeking for an explanation about genetically engineered Glycine max (soybean). During the project I was following, CRISPR-Cas9 mediated FAD2-2 gene deletion was performed on the plant seed. During sgDNA design though, it was done a blast of G.max FAD2-2 against Phsycomitrella patens FAD2-2. Since the similarities were high (99%) , the sgRNA was designed based on P. patiens sequence. Why sgRNA is preferred to be designed based on P. patens FAD2-2 sequence? |