Question about electrophoresis of GAPDH-fragment - Printable Version +- Biotechnology Forums (https://www.biotechnologyforums.com) +-- Forum: Information (https://www.biotechnologyforums.com/forum-16.html) +--- Forum: Questions and Answers (https://www.biotechnologyforums.com/forum-37.html) +--- Thread: Question about electrophoresis of GAPDH-fragment (/thread-8401.html) |
Question about electrophoresis of GAPDH-fragment - Shinu - 06-07-2018 Hi everyone! I have a question about an electrophoresis of the GAPDH-fragment. We made a master mix with the following: - Puffer Y - dNTPs - Primer forward & reverse - Taq Polymerase - DNA template - PCR water Now I got the 1 band (x) which I don't know what it is. I thought of mutations probably or the remaining DNA-template that didn't get cut as it should. (Picture is attached) Does anyone know what it might be? Thanks in advance!!=) |