+- Biotechnology Forums (https://www.biotechnologyforums.com)
+-- Forum: Information (https://www.biotechnologyforums.com/forum-16.html)
+--- Forum: Questions and Answers (https://www.biotechnologyforums.com/forum-37.html)
+--- Thread: Gene analysis - Identification homologous genes - Need Answer plz!! (/thread-2620.html)
Gene analysis - Identification homologous genes - Need Answer plz!! - ifhmn8 - 11-13-2013
What are the steps in identification homologous genes between Arabidopsis and tomato. I need to identify the homologous genes in tomato, do multiple alignment, build phylogenetic tree, and find analyze synteny.
2) What kind of alignment should I used, NJ or ML?
3) How to identify synthenic block for my gene of interest
4) is it acceptable if I BlastP the amino acid sequence from Arabidopsis as a query and the first hit has E-value of 9e-19 and 42% of identity?