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+--- Thread: Enzyme technology ( catechin assay) (/thread-1545.html)
Enzyme technology ( catechin assay) - angelllee0113 - 03-18-2012
Well, I'm using catechin for my polyphenol oxidase assay to determine the substrate specificity. But the problem is when I try to use 50mM above, the concentration seems to be very high which the spectrophotometer shows more than 4.500. What should I do?? I couldn find any info from the website or journals. Should I dilute it? And I should dilute the substrate only or together with the enzyme? Please help me ! Thanks a lot !!
RE: Enzyme technology ( catechin assay) - angelllee0113 - 03-18-2012
Please please anyone ?? Its for my final year project =((
RE: Enzyme technology ( catechin assay) - genhunter - 03-27-2012
Yes. You need to dilute it to a linear range to measure the product concentration.