Biotechnology Categories - Printable Version +- Biotechnology Forums (https://www.biotechnologyforums.com) +-- Forum: Biotechnology Discussion (https://www.biotechnologyforums.com/forum-1.html) +--- Forum: Main Biotechnology Discussion Forum (https://www.biotechnologyforums.com/forum-2.html) +--- Thread: Biotechnology Categories (/thread-1503.html) |
Biotechnology Categories - Capkeeds - 01-03-2012 Hi This forum provide all kinds of biotechnology categories information which related to the human health. http://www.chiropracticnewsletter.com/blog/chiropractic-newsletter RE: Protein Isolation from HL1 cell lines - bujjibabu12 - 08-09-2012 Dear all, I am Bujjibabu, I am working as research assistant in Korea. I am doing Protein isolation from HL1 cell lines. I did not get bands after western analysis. I am using Drugs; KN93-----30nM----------30min (inhibitor) ET-1-----10nM----------10min (receptor) primary abs; alpha actinine 1:5000 CREB 1:1000 phaspo CREB 1:1000 gel percentage 10% and concentration 30 micro grams sample Actually I am new for this area, Please anyone can answer my problem. I look forward to your replays. Regards, Bujjibabu. |