Biotechnology Forums
Biotechnology Categories - Printable Version

+- Biotechnology Forums (https://www.biotechnologyforums.com)
+-- Forum: Biotechnology Discussion (https://www.biotechnologyforums.com/forum-1.html)
+--- Forum: Main Biotechnology Discussion Forum (https://www.biotechnologyforums.com/forum-2.html)
+--- Thread: Biotechnology Categories (/thread-1503.html)



Biotechnology Categories - Capkeeds - 01-03-2012

Hi

This forum provide all kinds of biotechnology categories information which related to the human health.
http://www.chiropracticnewsletter.com/blog/chiropractic-newsletter


RE: Protein Isolation from HL1 cell lines - bujjibabu12 - 08-09-2012

Dear all,
I am Bujjibabu, I am working as research assistant in Korea.
I am doing Protein isolation from HL1 cell lines. I did not get bands after western analysis.
I am using Drugs;
KN93-----30nM----------30min (inhibitor)
ET-1-----10nM----------10min (receptor)
primary abs; alpha actinine 1:5000
CREB 1:1000
phaspo CREB 1:1000
gel percentage 10% and concentration 30 micro grams sample

Actually I am new for this area, Please anyone can answer my problem.

I look forward to your replays.

Regards,
Bujjibabu.