06-21-2018, 11:36 PM
Hi, i'm a bachelor student seeking for an explanation about genetically engineered Glycine max (soybean).
During the project I was following, CRISPR-Cas9 mediated FAD2-2 gene deletion was performed on the plant seed.
During sgDNA design though, it was done a blast of G.max FAD2-2 against Phsycomitrella patens FAD2-2. Since the similarities were high (99%) , the sgRNA was designed based on P. patiens sequence.
Why sgRNA is preferred to be designed based on P. patens FAD2-2 sequence?
During the project I was following, CRISPR-Cas9 mediated FAD2-2 gene deletion was performed on the plant seed.
During sgDNA design though, it was done a blast of G.max FAD2-2 against Phsycomitrella patens FAD2-2. Since the similarities were high (99%) , the sgRNA was designed based on P. patiens sequence.
Why sgRNA is preferred to be designed based on P. patens FAD2-2 sequence?