Biotechnology Forums

Hello every one,

I've done Ames test to see the mutagenicity in some vegetables that were grown using treated waste water ..

I took 5 gm of each vegetable and homogenized it with 100ml DH2O == (5gm/100ml)
the vegetable juice was filtered and 100Ml was taken to be grown with the top agar then incubated in 37C for 48 hr
the control was Sodium Azide with the S.typhi TA100 and the colony count was for the control (486 CFU)
I know that the colony count for the +ve control should be more than 1000CFU for the Sodium azide with TA100 , but this is the best result that i had in many trials.
now I'm trying to calculate the mutagenicity % for my results and I need to consider if I had 100gm of my vegetables

I konw that the main formula is:

The % mutagenicity as positive control :
(# colony plate/+ve control )*100%

for example :
(34/486)*100= 6.99 (this number in 5gm/100ml)
but if I need it /100gm
should I multiply it with 20 (as 100/5) or multiply it with 100??

& is my calculations are right or what??

sorry for the long message
thanx

hellooooooo ppl
is there is anyone can help in this???