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GATE BT-2017 Answer Key | Solutions Discussion
(02-16-2017, 01:48 AM)CHANDA7 Wrote:
(02-16-2017, 01:33 AM)Manoj_Shastry Wrote: EcoRI restriction sites on 10Kb DNA is shown.
Upon partial digestion, what are lengths of all possible DNA fragments obtained (in kb)? 
[Image: 585_217875_0_587434_r_bt_q51.jpg]
I choose option 1
can u please explain...
(03-02-2017, 06:10 AM)kashyap.aluru Wrote:
(02-16-2017, 01:48 AM)CHANDA7 Wrote: I choose option 1
can u please explain...
When partial digestion takes place, the enzyme may cleave at each and every possible restriction site..... If the enzyme cleaves at only the first restriction site, you can get a 8 kb fragment....
(03-02-2017, 06:10 AM)kashyap.aluru Wrote:
(02-16-2017, 01:48 AM)CHANDA7 Wrote: I choose option 1
can u please explain...
(03-02-2017, 01:52 AM)RahulK Wrote: We have found 3 questions till now:

1. Pedigree (question-45)  (Possible Ans : "X- linked recessive inheritance or Y linked" and "Y-linked only") (Can give upto 2.66 marks)
2. Zero order rate kinetics (question- 49) (Possible Ans : "100" and "10000") (Can give upto 2 marks)
3. Cytokinesis inhibitor (question -18)  (Possible Ans : "Cytochalasin D" and "Colchicine" ) (Can give upto 1.33 marks)

These 3 questions have possibility of giving a student 6 marks which can be really crucial.

Each of the 2 answers in the above question is proving to be right, thus all the students who have answered any of the 2 possible answers in the above questions should be given the benefit of doubt.

What is views from all of you guys, should we challenge all the 3 questions ?

For the Pedigree question: 

I found the below images from book :

Genetics : A conceptual Approach by Benjamin A Pierce

Image 1: Shows the standard symbols used in pedigree chart

Image 2: Shows a pedigree chart which is somewhat similar to the gate question pedigree chart

Besides that 

I went through a video on NPTEL course "Human Molecular Genetics" which is on pedigree analysis at this link :


http://nptel.ac.in/courses/102104052/5

Between minute 32 and 33 of the video professor says that the carriers in "x linked recessive condition" are denoted by a "dot within the circle". 
We can challenge the question giving the reason that no clear indication is given if any of the females in the given pedigree are carriers thus X linked recessive condition was not indicative from the given pedigree. 

I think these should make a strong case for review of Pedigree question and hold in favour of us because it is in the NPTEL cource lecture as well as a standard book.

Smile Smile

Image(s)
       
(03-02-2017, 02:49 PM)RahulK Wrote:
(03-02-2017, 01:52 AM)RahulK Wrote: We have found 3 questions till now:

1. Pedigree (question-45)  (Possible Ans : "X- linked recessive inheritance or Y linked" and "Y-linked only") (Can give upto 2.66 marks)
2. Zero order rate kinetics (question- 49) (Possible Ans : "100" and "10000") (Can give upto 2 marks)
3. Cytokinesis inhibitor (question -18)  (Possible Ans : "Cytochalasin D" and "Colchicine" ) (Can give upto 1.33 marks)

These 3 questions have possibility of giving a student 6 marks which can be really crucial.

Each of the 2 answers in the above question is proving to be right, thus all the students who have answered any of the 2 possible answers in the above questions should be given the benefit of doubt.

What is views from all of you guys, should we challenge all the 3 questions ?

For the Pedigree question: 

I found the below images from book :

Genetics : A conceptual Approach by Benjamin A Pierce

Image 1: Shows the standard symbols used in pedigree chart

Image 2: Shows a pedigree chart which is somewhat similar to the gate question pedigree chart

Besides that 

I went through a video on NPTEL course "Human Molecular Genetics" which is on pedigree analysis at this link :


http://nptel.ac.in/courses/102104052/5

Between minute 32 and 33 of the video professor says that the carriers in "x linked recessive condition" are denoted by a "dot within the circle". 
We can challenge the question giving the reason that no clear indication is given if any of the females in the given pedigree are carriers thus X linked recessive condition was not indicative from the given pedigree. 

I think these should make a strong case for review of Pedigree question and hold in favour of us because it is in the NPTEL cource lecture as well as a standard book.

Smile Smile
Yes u r right.
  

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