Genetically engineered papaya - agrobacterium or gene gun?

[Sinaeh]

Hello!

I am writing a paper on the genetically modified papaya (55-1), engineered to contain a coat protein gene of the papaya ringspot virus.. As far as I understand, this gene is used for cross protection (use of a mild virus variety to protect the plant against damage caused by the more severe variety of the same virus)

Here is my question! I have to explain the process of how it was engineered, and so far found information that describes how a agrobacterium tumefaciens plasmid was used, containing the coat protein and a few antibiotic selectable markers. However, in the same paragraph, I also read that the plasmid DNA was added to the papaya genome by "particle bombardment" - which would be the use of a gene gun.

So, is it possible to use both methods or am I misunderstanding the process?

Also, could someone explain to me what exactly promotors and cassettes are and how they are used to engineer the plant?


Thank you so much!

Lotte

[Payton]

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