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Thread: Meat from vegetable
Post: RE: Meat from vegetable
I think I understand your question a bit better now. You are not trying to create a product that tastes like meat or has the consistency of meat. You are trying to create a nutritionally balanced pl...
Author: walkingfish
01-26-2016, 08:21 AM
Thread: Meat from vegetable
Post: RE: Meat from vegetable
There are two different issues that you bring up: One is an issue of creating specific protein(s), and the other is creating "meat". "Meat" is really a culinary term, not a scientific one, and descr...
Author: walkingfish
12-21-2015, 04:08 AM
Thread: bioreactor anti-foam solutions
Post: bioreactor anti-foam solutions
I have noticed that problems of foam in bioreactors seem to be addressed in one of two ways: Either there is a foam detector that, when it detects foam, dispenses an anti-foam agent into the bioreact...
Author: walkingfish
12-20-2015, 06:01 PM
Thread: embryonic stem cell
Post: RE: embryonic stem cell
I sure am not going to open the file. I don't know what malware it might contain. If you want an answer, I'm sure you can at least post the questions, instead of creating more work for those that mi...
Author: walkingfish
11-15-2015, 03:57 PM
Thread: Cryptochrome in drosophila
Post: RE: Cryptochrome in drosophila
Check out wikipedia. They have an article on it.
Author: walkingfish
11-15-2015, 03:55 PM
Thread: Biological Characterization
Post: RE: Biological Characterization
Are trying to build a machine that does tests? I don't know what you are trying to do. If you are wondering about testing cytotoxicity of a chemical, etc., there are all sorts of ways. Toxins might...
Author: walkingfish
11-15-2015, 03:51 PM
Thread: multiple expression
Post: RE: multiple expression
This might seem like a simple question, but answering it involves a great deal of knowledge, much of which is unknown. In the case of sugar production, the "specific" gene(s) will differ from organis...
Author: walkingfish
11-15-2015, 03:28 PM
Thread: Size of laminar flow hood (bioreactor culture)
Post: Size of laminar flow hood (bioreactor culture)
I'm trying to get some idea as to parameter specs for cell culture.  Thus far, people here have been very helpful, and I'm very appreciative.  As a working standard, I'm envisioning using a bioreactor...
Author: walkingfish
11-15-2015, 12:28 PM
Thread: opening a bioreactor to put a scaffold in
Post: opening a bioreactor to put a scaffold in
Let's say that a bioreactor has been filled with media and autoclaved.  At this point, if I wanted to open it (ideally through a port, rather than taking the entire top off) for several seconds under ...
Author: walkingfish
11-09-2015, 03:03 PM
Thread: In cell culture, can treated surface survive autoclaving?
Post: In cell culture, can treated surface survive autoc...
If I treat a surface for cell adhesion with fibronectin, collagen, etc., can I autoclave it and expect it to function in allowing the attachment of mammalian cells?  Since denatured collagen is used t...
Author: walkingfish
11-09-2015, 02:45 PM
Thread: Fetal Bovine Serum questions
Post: Fetal Bovine Serum questions
The following relates to cell culture:   It is my understanding that FBS (fetal bovine serum) is preferable to calf serum or adult bovine serum for two reasons.  The first reason is that FBS contains ...
Author: walkingfish
10-22-2015, 09:46 AM