Standardization of qPCR gene expression data from different biological replicates - Printable Version +- Biotechnology Forums (https://www.biotechnologyforums.com) +-- Forum: Information (https://www.biotechnologyforums.com/forum-16.html) +--- Forum: Questions and Answers (https://www.biotechnologyforums.com/forum-37.html) +--- Thread: Standardization of qPCR gene expression data from different biological replicates (/thread-7829.html) |
Standardization of qPCR gene expression data from different biological replicates - nayani20 - 03-03-2017 Hey, I have performed three experiments on gene expression studies of drug treated and untreated samples of lung cancer cell lines to quantify the expression profile of 11 genes with 1 reference gene using quantitative real time PCR. This was performed with three biological replicates and two technical replicates for each biological replicate. I have observed a general trend in the regulation of the genes. However, these three experiments were performed on three different plates and so I would like to know how to compile the results from all the three experiments to reach a proper conclusion. How do I perform a statistical analysis of the data? |