Fingerprinting in Tissue Culture - Printable Version +- Biotechnology Forums (https://www.biotechnologyforums.com) +-- Forum: Information (https://www.biotechnologyforums.com/forum-16.html) +--- Forum: Questions and Answers (https://www.biotechnologyforums.com/forum-37.html) +--- Thread: Fingerprinting in Tissue Culture (/thread-7029.html) |
Fingerprinting in Tissue Culture - CandyWangui - 06-22-2015 How can one use finger printing in tissue culture to ensure genomic consistency, for a commercial lab that is? Tissue culture - CandyWangui - 06-22-2015 Which are the basic protocols in micropropagation of Eucalyptus, Camellia sinensis and Coffea Arabica of initiating in vitro considering phenolics production? Conditions for culturing other than initiating in darkness at 25 degrees Celsius. Avoiding Phenolics in Micropropagation, Tissue Culture - stevewoods - 06-22-2015 Hi Candy, I assume you want to avoid the production of phenolics (as they lead to necrosis)? For Eucalyptus, as read in many papers, subculturing seems an answer (though not a perfect answer) to phenolics. A useful review on Eucalyptus micropropagation suggested following to avoid phenolics in tissue culture of Eucalyptus (pasted verbatum):
Refer the review here: http://treephys.oxfordjournals.org/content/9/4/435.full.pdf I am sure some of these would provide answers to Tea and Coffee micropropagation as well. Hope you went through the very old paper of Koretzkaya? If you find some other suitable solution for dealing with phenolics, do let me know. RE: Fingerprinting in Tissue Culture - stevewoods - 06-22-2015 (06-22-2015, 08:54 PM)CandyWangui Wrote: How can one use finger printing in tissue culture to ensure genomic consistency, for a commercial lab that is? Can you elaborate on your question a bit? Sorry couldn't get the jest of it.. |