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RE: GATE BT 2016 - Taniya - 08-29-2015 sir, how should i study microbiology part? RE: GATE BT 2016 - dipak0mukherjee - 09-06-2015 sir, iisc has published the syllabus for gate 2016 biotechnology..analytical techniques ..this part has been added..which book should i follow for this part? in biochemistry neucleotide metabolism ,amino acid metabolism ,lipid metabolism are also added...:-( and in the part of bioprocess engineering unit operations in liquid liquid and solid liquid has been added..which book should i follow for this ? BT Biotechnology (NEW SYLLABUS) Section 1: Engineering Mathematics Linear Algebra: Matrices and determinants, Systems of linear equations, Eigen values and Eigen vectors. Calculus: Limit, continuity and differentiability, Partial derivatives, Maxima and minima, Sequences and series, Test for convergence, Fourier Series. D ifferential Equations: Linear and nonlinear first order ODEs, higher order ODEs with constant coefficients, Cauchy’s and Euler’s equations, Laplace transforms, PDE - Laplace, heat and wave equations. Probability and Statistics: Mean, median, mode and standar d deviation, Random variables, Poisson, normal and binomial distributions, Correlation and regression analysis. Numerical Methods: Solution of linear and nonlinear algebraic equations, Integration of trapezoidal and Simpson’s rule, Single and multistep met hods for differential equations. Section 2: General Biotechnology Biochemistry: Biomolecules - structure and functions; Biological membranes, structure, action potential and transport processes; Enzymes - classification, kinetics and mechanism of action; Basi c concepts and designs of metabolism (carbohydrates, lipids, amino acids and nucleic acids) photosynthesis, respiration and electron transport chain; Bioenergetics Microbiology : Viruses - structure and classification ; Microbial classification and diversity(bacterial, algal and fungal) ; Methods in microbiology ; Microbial growth and nutrition ; Aerobic and anaerobic respiration ; Nitrogen fixation ; Microbial diseases and host - pathogen interaction Cell Biology : Prokaryotic and eukaryotic cell structure ; Cell cycle and cell growth control ; Cell - Cell communication, Cell signaling and signal transduction Molecular Biology and Genetics : Molecular structure of genes and chromosomes; Mutations and mutagenesis; Nucleic acid replication, transcription, transla tion and their regulatory mechanisms in prokaryotes and eukaryotes; Mendelian inheritance; Gene interaction; Complementation; Linkage, recombination and chromosome mapping; Extra chromosomal inheritance; Microbial genetics (plasmids, transformation, transd uction, conjugation); Horizontal gene transfer and Transposable elements; RNA interference; DNA damage and repair; Chromosomal variation; Molecular basis of genetic diseases Analytical Techniques: Principles of microscopy - light, electron, fluorescent and confocal ; Centrifugation - high speed and ultra; Principles of spectroscopy - UV, visible, CD, IR, FTIR, Raman, MS,NMR ; Principles of chromatography - ion exchange, gel filtration, hydrophobic interaction, affinity , GC,HPLC, FPLC; Electrophoresis ; Microarray Immunology : History of Immunology; Innate, humoral and cell mediated immunity; Antigen ; Antibody structure and function ; Molecular basis of antibody diversity; Synthesis of antibody and secretion; Antigen - antibody reaction; Complement ; Primary and secon dary lymphoid organ; B and T cells and macrophages ; Major histocompatibility complex (MHC) ; Antigen processing and presentation ; Polyclonal and monoclonal antibody ; Regulation of immune response; Immune tolerance ; Hypersensitivity; Autoimmunity; Graft vers us host reaction. Bioinformatics: Major bioinformatic resources and search tools; Sequence and structure databases ; Sequence analysis (biomolecular sequence file formats, scoring matrices, sequence alignment, phylogeny);Data mining and analytical tools for genomic and proteomic studies; Molecular dynamics and simulations (basic concepts including force fields, protein - protein, protein - nucleic acid, protein - ligand interaction) Section 3: Recombinant DNA Technology Restriction and modification enzymes; Vectors; plasmid, bacteriophage and other viral vectors, cosmids, Ti plasmid, yeast artificial chromosome; mammalian and plant expression vectors; cDNA and genomic DNA library; Gene isolation, cloning and expression ; Transposons and gene targeting; DNA labeling; DNA sequencing; Polymerase chain reactions; DNA fingerprinting; Southern and northern blotting; In - situ hybridization; RAPD, RFLP; Site - directed mutagenesis; Gene transfer technologies; Gene therapy Section 4: Plant and An imal Biotechnology Totipotency; Regeneration of plants; Plant growth regulators and elicitors; Tissue culture and Cell suspension culture system: methodology, kinetics of growth and, nutrient optimization; Production of secondary metabolites by plant susp ension cultures; Hairy root culture; transgenic plants; Plant products of industrial importance Animal cell culture; media composition and growth conditions; Animal cell and tissue preservation; Anchorage and non - anchorage dependent cell culture; Kinetics of cell growth; Micro & macro - carrier culture; Hybridoma technology; Stem cell technology; Animal cloning; Transgenic animals Section 5: Bioprocess Engineering and Process Biotechnology Chemical engineering principles applied to biological system, Princip le of reactor design, ideal and non - ideal multiphase bioreactors, mass and heat transfer; Rheology of fermentation fluids, Aeration and agitation; Media formulation and optimization; Kinetics of microbial growth, substrate utilization and product formation ; Sterilization of air and media; Batch, fed - batch and continuous processes; Various types of microbial and enzyme reactors; Instrumentation control and optimization; Unit operations in solid - liquid separation and liquid - liquid extraction; Process scale - up , economics and feasibility analysis Engineering principle of bioprocessing - Upstream production and downstream; Bioprocess design and development from lab to industrial scale; Microbial, animal and plant cell culture platforms; Production of biomass and primary/secondary metabolites; Biofuels, Bioplastics, industrial enzymes, antibiotics; Large scale production and purification of recombinant proteins; Industrial application of chromatographic and membrane based bioseparation methods; Immobilization of bi ocatalysts (enzymes and cells) for bioconversion processes; Bioremediation - Aerobic and anaerobic processes for stabilization of solid / liquid wastes RE: GATE BT 2016 - SunilNagpal - 09-07-2015 Hi...i am out of station these days...will need to go through your post thoroughly as I settle with a good internet connection...responding using my phone as of now....as far as analytical techniques go, Wilson and Walker's book is the best for that...I followed the same during my BTech as well as Masters....Doran has always been my first and most recommended book for bioprocess...you can find all unit operations in Doran....shall get in touch with detailed suggestions soon.. RE: GATE BT 2016 - dipak0mukherjee - 09-08-2015 thank u so much sir... Start of preparation for GATE 2016 - aadhijk5 - 09-10-2015 Dear Sir, Could you please tell me how am I to start the preparation of GATE 2016? RE: GATE BT 2016 - Palak Sangal - 09-13-2015 Hello Sir, This year IISc Bangalore is the organising institute for GATE. Concerning the organising institute can you please tell which subjects are most important ! RE: GATE BT 2016 - prakash choudhary - 09-19-2015 Dear Sir , I am student of btech biotech preaparing for gate , I have a doubt that I am preaparing from path finder , arihant and path finder MCQ , so sir I think it is not sufficient so sir can you provide any idea that i can prepared in more better way. RE: GATE BT 2016 - Sharmin Calcuttawala - 11-10-2015 Sir I m not able to solve the 51 question of gate 2011 biotechnology paper . Sir plz do help me wid the 51st sum RE: GATE BT 2016 - dipak0mukherjee - 11-28-2015 SIR, need hints to solve a sum.. animal cells are growing in a 5 m^3 CSTR with blood serum as feed.feed substrate concentration,S0= 20 KG/M^3,u MAX=0.45 HR^-1,Ks=0.8 kg/m^3,Yx/s=0.8.what feed flow rate is required to achieve 90% conversion of the substratre RE: GATE BT 2016 | Bioprocess Previous Year Solved Question - SunilNagpal - 12-03-2015 (11-28-2015, 01:47 AM)dipak0mukherjee Wrote: SIR,I am not sure about the solution, but this should be fine: So = 20 S = 20 x 0.1 = 2 (90% conversion means 10% residual): i.e steady state concentration of the substrate in chemostat Umax=0.45 hr-1 Ks = 0.8 V=5 We know, Dilution rate = Flow rate / Volume And, we also know that Dc (Critical dilution rate) = Umax ( S/ Ks+ S) So, Dc = 0.45 x 2 / 2.8 = 0.9 / 2.8 So, this should give us flow rate = (0.9/2.8) x 5 = 1.607 m3/hr (Options might give a better hint towards the right approach). Best wishes |