Biotechnology Forums

Dear Members,


This is a dedicated thread for discussions pertaining to CSIR JRF/ UGC NET. Feel free to post queries, tips and suggestions here.

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Starting with the query of forum member: CHANDA7:

This is a question from CSIR NET 2016 December..... Can anyone help me with the question number 142?

[attachment=400]

(03-06-2017, 07:30 PM)SunilNagpal Wrote: [ -> ]Dear Members,


This is a dedicated thread for discussions pertaining to CSIR JRF/ UGC NET. Feel free to post queries, tips and suggestions here.

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Starting with the query of forum member: CHANDA7:

This is a question from CSIR NET 2016 December..... Can anyone help me with the question number 142?

#CHANDA7
Best possible answer is 2. Protein is in insoluble/pellet because its getting denatured at that particular temperature. Lowering temperature will prevent this and solublize the protein

#Chanda7

Apologize. I used the Set A question paper. Image did not load.

I think the picture below summarizes. If it had a correct orientation(1st plasmid) - BamHI + HindIII  == 0.5 + 4.5
                                                                                                                              PstI    + HindIII   == 1.5 + 3.5
Answer is C. It will be reversed(A) for incorrect orientation. 

(03-06-2017, 10:39 PM)travellingsalesman Wrote: [ -> ]#Chanda7

Apologize. I used the Set A question paper. Image did not load.

I think the picture below summarizes. If it had a correct orientation(1st plasmid) - BamHI + HindIII  == 0.5 + 4.5
                                                                                                                              PstI    + HindIII   == 1.5 + 3.5
Answer is C. It will be reversed(A) for incorrect orientation. 

Thanks bro..... I didn't think it's so easy

Can anyone help me with this question?
( For search: IPTG, T7 RNA polymerase, lac operator, cell free supernatant)

[attachment=404] can anybody help me with these two questions?

(03-08-2017, 03:56 PM)CHANDA7 Wrote: [ -> ]Can anyone help me with this question?
( For search: IPTG, T7 RNA polymerase, lac operator, cell free supernatant)

I answered this question before(accidently)
Best possible answer is 2. Protein is in insoluble/pellet because its getting denatured at that particular temperature. Lowering temperature will prevent this and solublize the protein

(03-28-2017, 09:41 PM)travellingsalesman Wrote: [ -> ]

(03-08-2017, 03:56 PM)CHANDA7 Wrote: [ -> ]Can anyone help me with this question?
( For search: IPTG, T7 RNA polymerase, lac operator, cell free supernatant)

I answered this question before(accidently)
Best possible answer is 2. Protein is in insoluble/pellet because its getting denatured at that particular temperature. Lowering temperature will prevent this and solublize the protein

Thanks bro..... Can u explain what could be the expected changes as of other options?

[attachment=407] can anybody answer this with justification???
Please help me.....

Key for search: Circular Dichroism, psychrophile, thermophile, protein....

May be the answer is : d.. bcz of salt bridge made of by the hydrogen bond and we knw H- bonding highly stable...for that reason last one is the answer...what is the answer???