Biotechnology Forums

What are all the applications of s1 mapping? Can it anyway be used in forensics?

Nuclease s1 will digest only ssDNA and ssRNA.. if we consider in forensic science, during DNA fingerprinting we may use it to digest ssDNA overhangs so that when we run the sample through gel in electrophoresis... no complication will arise. .

S1 mapping of an intron site. Nuclease digest the unhybridized intronic DNA which is not present in RNA.

Thanks for the info. But during DNA fingerprinting what will be used as the probe?