05-10-2016, 12:16 AM
Has anyone ever tried to produce MAb with a peptide (20 a.a sequence) with FCA adjuvant alone, without any carrier protein. Does it work, If so could you direct me to a paper?
the other group tried KLH conjugate. I'am trying without carrier protein with adjuvant, I don't see nice titer values. Is this even a feasible approach.
people had a problem with carrier that antibodies were raised against the carrier and very low against desired epitope. Is there any paper that worked out peptide without carrier. I see in every book they mention associating with carrier protein .
the other group tried KLH conjugate. I'am trying without carrier protein with adjuvant, I don't see nice titer values. Is this even a feasible approach.
people had a problem with carrier that antibodies were raised against the carrier and very low against desired epitope. Is there any paper that worked out peptide without carrier. I see in every book they mention associating with carrier protein .